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-Structure paper
| タイトル | Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulation. |
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| ジャーナル・号・ページ | Nat Commun, Vol. 17, Issue 1, Page 693, Year 2025 |
| 掲載日 | 2025年12月13日 |
著者 | Johann J Roske / Giulia Paris / Akanksha Goyal / Marina Rodnina / Nikolay Zenkin / Katarzyna J Bandyra / Ben F Luisi / ![]() |
| PubMed 要旨 | In many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from ...In many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from cryo-EM demonstrating that a bacterial RNA polymerase that is paused proximally to the promoter can associate with the pioneering 30S translation initiation complex (30S IC). These findings suggest that the physical link between transcription and translation can be established prior to commitment to protein synthesis. Although the mRNA is embedded in this 'early expressome' complex, it can nonetheless interact with small regulatory RNA (sRNA) and be targeted for cleavage in the protein-coding region by the RNA degradosome assembly in vitro. The potential tagging of transcripts with sRNA during pioneering and subsequent stages of translation initiation, when the 30S IC is at the 5' end of a polyribosome, may in principle contribute to efficient and rapid termination of gene expression in response to regulatory signals. |
リンク | Nat Commun / PubMed:41390511 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.0 - 5.8 Å |
| 構造データ | ![]() EMDB-55527: Early Expressome Composite Map of TEC and 30S IC ![]() EMDB-55528: Early Expressome Consensus Refinement ![]() EMDB-55529: Early Expressome RNAP/TEC body ![]() EMDB-55530: E. coli 30S IC containing mRNA, IF1 and IF3 ![]() EMDB-55531: E. coli 30S IC containing mRNA, initiator tRNA, IF1 and IF2 |
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