Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | chromatin dynamics and HIV-1 nuclear trafficking. |
|---|---|
| Journal, issue, pages | bioRxiv, Year 2025 |
| Publish date | Dec 15, 2025 |
 Authors | Zhen Hou / Yao Shen / Long Chen / Juan Shen / David I Stuart / Peijun Zhang /  |
| PubMed Abstract | The organization and dynamics of chromatin within intact nuclei remain poorly defined, limiting understanding of how nuclear architecture influences macromolecular transport. HIV-1 provides a ...The organization and dynamics of chromatin within intact nuclei remain poorly defined, limiting understanding of how nuclear architecture influences macromolecular transport. HIV-1 provides a striking example of a large complex that must traverse this dynamic landscape to access integration sites within transcriptionally active regions. Here, we combine a functional nuclear import system with correlative cryo-electron tomography to visualize chromatin architecture and HIV-1 cores during nuclear penetration. Native nucleosomes resolved at 5.6 Å reveal four structural classes, from compact conformations at the nuclear periphery to open, flexible forms in the interior, defining a spatially heterogeneous and dynamic chromatin landscape. Within this environment, HIV-1 cores decorated with nuclear factors exclude nearby nucleosomes and preferentially associate with open chromatin. Disruption of CPSF6-capsid interactions abolishes these associations, confining viral cores to peripheral chromatin. These findings establish an framework linking chromatin structure and dynamics to HIV-1 nuclear trafficking and provide a structural basis for viral navigation within the dynamic nuclear landscape. |
 External links | bioRxiv / PubMed:41446222 / PubMed Central |
| Methods | EM (subtomogram averaging) |
| Resolution | 5.6 - 20.0 Å |
| Structure data |  EMDB-55441: In situ structure of wild-type HIV-1 CA hexamer prior to nuclear import  EMDB-55443: In situ structure of wild-type HIV-1 CA hexamer post nuclear import  EMDB-55445: In situ structure of N74D HIV-1 CA hexamer post nuclear import  EMDB-55446: In situ structure of the H1-bound nucleosome  EMDB-55447: In situ structure of stacking H1-bound nucleosomes  EMDB-55448: In situ structure of the H1-bound nucleosome in stacking nucleosomes  EMDB-55449: In situ structure of the core nucleosome  EMDB-55450: In situ structure of the open-linker H1-bound nucleosome |
| Source |
|
Human immunodeficiency virus 1
Homo sapiens (human)