Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of Mlc-mediated transcriptional regulation of carbohydrate metabolism.
Journal, issue, pages	Nat Commun, Year 2026
Publish date	Sep 25, 2025
Authors	Patrick Roth / Inken Fender / Jean-Marc Jeckelmann / Zöhre Ucurum / Thomas Lemmin / Dimitrios Fotiadis /
PubMed Abstract	The global transcriptional repressor Mlc of Escherichia coli regulates genes involved in carbohydrate transport and metabolism, particularly glucose uptake via the glucose-specific phosphotransferase ...The global transcriptional repressor Mlc of Escherichia coli regulates genes involved in carbohydrate transport and metabolism, particularly glucose uptake via the glucose-specific phosphotransferase system (PTS). Unlike conventional repressors, Mlc exemplifies a system in which interactions with diverse macromolecules govern its activity. Here, we present cryo-electron microscopy structures of Mlc alone and in complexes with regulatory partners, including the glucose-specific PTS transporter IICB, a cognate DNA operator and the anti-repressor MtfA, capturing multiple assemblies central to transcription control. These structures reveal the molecular architecture of Mlc and its interactions with binding partners. Together with molecular dynamics simulations, they provide insights into the structural dynamics of these complexes. Our findings establish the structural basis of membrane-transporter involvement in transcriptional regulation, the mechanism of anti-repressor action and DNA recognition. This work provides a structural framework for understanding bacterial transcriptional regulation across diverse systems.
External links	Nat Commun / PubMed:42436118
Methods	EM (single particle)
Resolution	2.3 - 2.96 Å
Structure data	EMDB Unreleased entry EMDB-55151: Focussed refinement of the Mlc tetramer Method: EM (single particle) / Resolution: 2.62 Å EMDB Unreleased entry EMDB-55152: Focused refinement of Mlc tetramer Method: EM (single particle) / Resolution: 2.89 Å 55153 EMDB Unreleased entry 9srt EMDB-55153, PDB-9srt: Cryo-EM structure of the Mlc tetramer Method: EM (single particle) / Resolution: 2.74 Å EMDB Unreleased entry EMDB-55154: Consensus reconstruction of Mlc tetramer in complex with ptsG operator DNA Method: EM (single particle) / Resolution: 2.47 Å EMDB Unreleased entry EMDB-55155: Focused reconstruction of Mlc tetramer in complex with ptsG operator DNA Method: EM (single particle) / Resolution: 2.56 Å 55156 EMDB Unreleased entry 9sru EMDB-55156, PDB-9sru: Cryo-EM structure of Mlc in complex with ptsG operator DNA Method: EM (single particle) / Resolution: 2.47 Å EMDB Unreleased entry EMDB-55157: Consensus reconstruction of the Mlc tetramer in complex with the anti-repressor MtfA Method: EM (single particle) / Resolution: 2.3 Å EMDB Unreleased entry EMDB-55158: Focused reconstruction of the Mlc tetramer in complex with the anti-repressor MtfA Method: EM (single particle) / Resolution: 2.3 Å EMDB Unreleased entry EMDB-55159: Focused reconstruction of the Mlc tetramer in complex with the anti-repressor MtfA Method: EM (single particle) / Resolution: 2.94 Å 55160 EMDB Unreleased entry 9srw EMDB-55160, PDB-9srw: Cryo-EM structure of the Mlc tetramer in complex with the anti-repressor MtfA Method: EM (single particle) / Resolution: 2.3 Å EMDB Unreleased entry EMDB-55161: Consensus reconstruction of the Mlc tetramer in complex with the glucose-specific IICB transporter Method: EM (single particle) / Resolution: 2.96 Å EMDB Unreleased entry EMDB-55162: Focused reconstruction of the Mlc tetramer in complex with the glucose-specific IICB transporter Method: EM (single particle) / Resolution: 2.78 Å EMDB Unreleased entry EMDB-55163: Focused reconstruction of the Mlc tetramer in complex with the glucose-specific IICB transporter Method: EM (single particle) / Resolution: 2.95 Å EMDB Unreleased entry EMDB-55164: Focused reconstruction of the Mlc tetramer in complex with the glucose-specific IICB transporter Method: EM (single particle) / Resolution: 2.81 Å 55165 EMDB Unreleased entry 9srx EMDB-55165, PDB-9srx: Cryo-EM structure of the Mlc repressor in complex with the glucose-specific IICB transporter Method: EM (single particle) / Resolution: 2.96 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-BGC: beta-D-glucopyranose
Source	escherichia coli (E. coli)
Keywords	DNA BINDING PROTEIN / Repressor / DNA-binding protein / tetramer / transcription regulation / gene regulation / carbohydrate utilization / metal-binding / DNA / operator / anti-repressor / Metalloprotease / Hydrolase / membrane transporter / glucose / transmembrane protein / membrane-tethering