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TitleDirect visualization and tracing of chromatin folding in the Drosophila embryo.
Journal, issue, pagesEMBO J, Year 2026
Publish dateFeb 9, 2026
AuthorsFadwa Fatmaoui / Pascal Carrivain / Fatima Taiki / Amina Iusupova / Diana Grewe / Wim Hagen / Burkhard Jakob / Jean-Marc Victor / Amélie Leforestier / Mikhail Eltsov /
PubMed AbstractChromatin organization, through the assembly of DNA with histones and the folding of nucleosome chains, regulates DNA accessibility for transcription, DNA replication and repair. Although models ...Chromatin organization, through the assembly of DNA with histones and the folding of nucleosome chains, regulates DNA accessibility for transcription, DNA replication and repair. Although models derived from in vitro studies have proposed distinct nucleosome chain geometries, the organization of chromatin within the crowded cell nucleus remains elusive. Using cryo-electron tomography of thin vitreous sections, we directly observed the path of nucleosomal and linker DNA in situ from a flash-frozen organism - Drosophila embryos. We quantified linker length and curvature, characterizing an irregular zig-zag chromatin-folding motif, with a low degree of linker bending. Nucleosome conformations could be identified on individual particles in favorable orientations without structure averaging. Additionally, we observed particles that accommodate a number of DNA gyres ranging from less than one to up to three, which resemble previously proposed non-octameric nucleosomal particles with variable DNA wrapping.
External linksEMBO J / PubMed:41663843
MethodsEM (subtomogram averaging)
Resolution20.0 Å
Structure data

EMDB-54985: Subtomogram average of nucleosomes extracted from vitreous sections of Drosophila melanogaster embryos
Method: EM (subtomogram averaging) / Resolution: 20.0 Å

Source
  • Drosophila melanogaster (fruit fly)

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