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TitleA combined biochemical and computational approach provides evidence for membrane remodelling by the structural scaffold of the endocytic TPLATE complex.
Journal, issue, pagesNat Plants, Vol. 11, Issue 11, Page 2423-2436, Year 2025
Publish dateNov 12, 2025
AuthorsJulia M Kraus / Michaela Neubergerová / Alvaro Furones Cuadrado / Neeltje Schilling / Dominique Eeckhout / Nancy De Winne / Eveline Van De Slijke / Michaël Vandorpe / Klaas Yperman / Evelien Mylle / Marcus Fislage / Geert De Jaeger / Roman Pleskot / Daniël Van Damme /
PubMed AbstractEukaryotic cells maintain homeostasis of their outer membrane by controlled internalization of lipid and protein constituents via endocytosis. Endocytosis is evolutionary conserved and uses similarly ...Eukaryotic cells maintain homeostasis of their outer membrane by controlled internalization of lipid and protein constituents via endocytosis. Endocytosis is evolutionary conserved and uses similarly folded domains. How these structural folds are combined into proteins and protein complexes, however, differs between eukaryotic kingdoms. The TPLATE complex (TPC) in plants is an evolutionary ancient protein module that combines several protein domains with a conserved role in endocytosis into a single octameric protein complex. Its molecular architecture, lipid-nucleated condensate formation and requirement for clathrin cage curvature revealed its function in endocytosis initiation in plants. Mechanistic understanding of how this complex drives membrane deformation during plant endocytosis is, however, lacking. Here we used an integrative structural approach to obtain a precise molecular structure of the TPC of Arabidopsis thaliana. In addition, our approach allowed visualizing the structural flexibility that hallmarks this enigmatic complex. We prove that the intrinsic structural flexibility is required for its functionality and membrane recruitment. The membrane-binding interface consists of several domains with differential lipid preferences. Finally, we demonstrate via molecular dynamics simulations that the crescent shape of the structured part of the complex is sufficient for membrane curvature generation. Our mechanistic insight, obtained by a combined biochemical and computational approach, shows that the structured part of the TPC likely contributes to the execution of plant endocytosis, which does not depend on cytoskeletal-based force generation.
External linksNat Plants / PubMed:41224962
MethodsEM (single particle)
Resolution17.0 Å
Structure data

EMDB-54584: Arabidopsis thaliana TPLATE complex negative stain EM map
Method: EM (single particle) / Resolution: 17.0 Å

Source
  • Arabidopsis thaliana (thale cress)

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