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TitleIn situ structure of a gap junction-stomatin complex.
Journal, issue, pagesSci Adv, Vol. 11, Issue 45, Page eaea8596, Year 2025
Publish dateNov 7, 2025
AuthorsNils Rosenkranz / Alexandra N Birtasu / Konstantin Wieland / Lisa Rehm / Rachita Sharma / Atal Vats / Sina Manger / Aayush Srivastava / Abhishek Bhattacharya / Gerhard Hummer / Achilleas S Frangakis / Alexander Gottschalk /
PubMed AbstractGap junctions (GJs) are intercellular channels that mediate electrical signals and transfer of small molecules. They are crucial for brain, heart, and other organ functions. While molecular ...Gap junctions (GJs) are intercellular channels that mediate electrical signals and transfer of small molecules. They are crucial for brain, heart, and other organ functions. While molecular structures of purified homomeric GJs are available, information of in situ structures is lacking. In vivo, GJs can form heteromers with different functionalities and may associate with other proteins. Here, we analyzed GJs by cryo-electron tomography and subtomogram averaging. We observed hexagonal arrays of GJs at cellular junctions in primary embryonal cells that displayed distinct wide and narrow conformations. Moreover, we found a cap-like, cytosolic protein assembly enclosing the channel pore. We propose that the cap is formed by the stomatin UNC-1, known to interact with UNC-9 innexins. This is corroborated by matching AlphaFold3 models of UNC-1 multimers with our subtomogram average structure; by expressing GFP-tagged UNC-1, leading to cap structures with additional density; and by coarse-grained MD simulations. UNC-1/stomatin rings may affect GJ formation or functions, possibly beyond nematodes.
External linksSci Adv / PubMed:41191766 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution15.0 - 25.6 Å
Structure data

EMDB-54522: C. elegans in situ Gap Junction class 1
Method: EM (subtomogram averaging) / Resolution: 15.0 Å

EMDB-54525: in situ Gap Junction C. elegans Class 2
Method: EM (subtomogram averaging) / Resolution: 18.0 Å

EMDB-54526: in situ C. elegans capped gap junction
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

EMDB-55045: Capped GJ with additional cytosolic Density
Method: EM (subtomogram averaging) / Resolution: 25.6 Å

Source
  • Caenorhabditis elegans (invertebrata)

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