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-Structure paper
| タイトル | Molecular basis for anti-jumbo phage immunity by AVAST type 5. |
|---|---|
| ジャーナル・号・ページ | Mol Cell, Vol. 86, Issue 4, Page 740-756.e9, Year 2026 |
| 掲載日 | 2026年2月19日 |
著者 | Aswin Muralidharan / Ana Rita Costa / Desi Fierlier / Daan Frits van den Berg / Halewijn van den Bossche / Adja Damba Zoumaro-Djayoon / Alicia Rodríguez-Molina / Martin Pabst / Martin Pacesa / Bruno E Correia / Stan J J Brouns / ![]() |
| PubMed 要旨 | Jumbo phages protect their genomes from DNA-sensing bacterial defense systems by enclosing them within vesicles and nucleus-like compartments. Very little is known about defense systems specialized ...Jumbo phages protect their genomes from DNA-sensing bacterial defense systems by enclosing them within vesicles and nucleus-like compartments. Very little is known about defense systems specialized to counter these phages. Here, we show that AVAST type 5 (Avs5) systems, part of the signal transduction ATPases of numerous domains (STAND) superfamily, confer conserved immunity against jumbo phages. Using fluorescence microscopy and biotin proximity labeling, we demonstrate that Avs5 localizes to early infection vesicles, where it senses an essential, early-expressed phage protein named JADA (Jumbo phage Avs5 Defense Activator). Recognition of phage infection triggers the Sir2-like effector domain of Avs5 across three Avs5 clades, resulting in rapid NAD hydrolysis, disruption of phage nucleus formation, and arrest of infection. These findings reveal a spatially coordinated bacterial immune strategy that targets an early vulnerability in jumbo phage infection. |
リンク | Mol Cell / PubMed:41653918 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.1 Å |
| 構造データ | EMDB-54139, PDB-9rp3: |
| 由来 |
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キーワード | UNKNOWN FUNCTION / jumbophage / gp316 / jada / avast5 / dimer |
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pseudomonas phage vb_pa32_gums (ファージ)
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