Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural and functional mapping of protective human monoclonal antibodies against enterovirus A71.
Journal, issue, pages	Sci Adv, Vol. 12, Issue 23, Page eaee8217, Year 2026
Publish date	Jun 5, 2026
Authors	Daming Zhou / Abhay Kotecha / James T Kelly / Peng-Nien Huang / Yi-Yin Chen / Thomas S Walter / Helen M E Duyvesteyn / Raymond J Owens / Shu-Yuan Ho / Tzou-Yien Lin / Elizabeth E Fry / Jingshan Ren / Kuan-Ying A Huang / David I Stuart /
PubMed Abstract	EV-A71 has been responsible for recent severe HFMD outbreaks. We report structures for 12 potently neutralizing human anti-EV-A71 monoclonal antibody Fabs, alone and complexed with virus. Most ...EV-A71 has been responsible for recent severe HFMD outbreaks. We report structures for 12 potently neutralizing human anti-EV-A71 monoclonal antibody Fabs, alone and complexed with virus. Most recognize the native antigenic state with epitopes that span interfaces, together covering 85% of the capsid surface. The majority (8 of 12) bind the canyon, while the others cluster around the icosahedral two- and threefold axes. Blocking SCARB2 receptor binding likely contributes to neutralization for all, and a subset induces empty particles. A predominant gene family (IGHV4-39) does not dictate a common binding pose. Long CDR-H3 loops are frequently key to binding, especially at the canyon, suggesting that antigenicity data based on antibodies with shorter CDR3s (e.g., murine) may be misleading. This dataset reveals neutralization mechanisms for recently circulating EV-A71 genotypes, which will inform immunotherapies. We demonstrate synergy in vitro between canyon binding and both two- and threefold binding antibodies to increase neutralization potency.
External links	Sci Adv / PubMed:42247493 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	1.14 - 4.8 Å
Structure data	53994 9rig EMDB-53994, PDB-9rig: EV-A71 (genotype B2) in complex with 16-2-9D Fab Method: EM (single particle) / Resolution: 3.7 Å 53995 9rih EMDB-53995, PDB-9rih: EV-A71 (genotype B2) in complex with 16-2-12D Fab Method: EM (single particle) / Resolution: 3.1 Å 53996 9rii EMDB-53996, PDB-9rii: EV-A71 (genotype B2) in complex with 34-1-6D Fab Method: EM (single particle) / Resolution: 3.0 Å 53997 9rij EMDB-53997, PDB-9rij: EV-A71 (genotype B5) in complex with 16-2-8C Fab Method: EM (single particle) / Resolution: 3.8 Å 53998 9rik EMDB-53998, PDB-9rik: EV-A71 (genotype B5) in complex with 16-2-2D Fab Method: EM (single particle) / Resolution: 3.4 Å 53999 9ril EMDB-53999, PDB-9ril: EV-A71 (genotype B5) in complex with 16-3-3C Fab Method: EM (single particle) / Resolution: 3.6 Å 54000 9rim EMDB-54000, PDB-9rim: EV-A71 (genotype B5) in complex with 16-3-4D Fab Method: EM (single particle) / Resolution: 3.0 Å 54001 9rin EMDB-54001, PDB-9rin: EV-A71 (genotype B5) in complex with 17-1-12A Fab Method: EM (single particle) / Resolution: 4.1 Å 54002 9rio EMDB-54002, PDB-9rio: EV-A71 (genotype B5) in complex with 17-2-2B Fab Method: EM (single particle) / Resolution: 3.4 Å 54003 9rip EMDB-54003, PDB-9rip: EV-A71 (genotype B5) in complex with 17-2-12A Fab Method: EM (single particle) / Resolution: 3.1 Å 54004 9riq EMDB-54004, PDB-9riq: EV-A71 (genotype C4) in complex with 16-2-11B Fab Method: EM (single particle) / Resolution: 3.3 Å 54005 9rir EMDB-54005, PDB-9rir: EV-A71 (genotype C4) in complex with 16-3-10B Fab Method: EM (single particle) / Resolution: 3.3 Å 55620 9t6z EMDB-55620, PDB-9t6z: EV-A71 (genotype B5) empty particle in complex with 16-3-3C Fab Method: EM (single particle) / Resolution: 4.8 Å PDB-9i3w: Structure of anti-EV71 human monoclonal antibody 16-2-8C Fab Method: X-RAY DIFFRACTION / Resolution: 1.14 Å PDB-9i3z: Structure of anti-EV71 human monoclonal antibody 16-2-12D Fab Method: X-RAY DIFFRACTION / Resolution: 3.06 Å PDB-9i40: Structure of anti-EV71 human monoclonal antibody 16-3-4D Fab Method: X-RAY DIFFRACTION / Resolution: 2.78 Å PDB-9i41: Structure of anti-EV71 human monoclonal antibody 16-3-3C Fab Method: X-RAY DIFFRACTION / Resolution: 1.32 Å PDB-9i42: Structure of anti-EV71 human monoclonal antibody 16-2-2D Fab Method: X-RAY DIFFRACTION / Resolution: 1.92 Å PDB-9i43: Structure of anti-EV71 human monoclonal antibody 16-2-9D Fab Method: X-RAY DIFFRACTION / Resolution: 1.18 Å PDB-9i45: Structure of anti-EV71 human monoclonal antibody 16-3-10B Fab Method: X-RAY DIFFRACTION / Resolution: 2.12 Å PDB-9i4b: Structure of anti-EV71 human monoclonal antibody 17-2-12A Fab Method: X-RAY DIFFRACTION / Resolution: 2.68 Å PDB-9i4c: Structure of anti-EV71 human monoclonal antibody 17-1-12A Fab Method: X-RAY DIFFRACTION / Resolution: 2.83 Å PDB-9i4d: Structure of anti-EV71 human monoclonal antibody 17-2-2B Fab Method: X-RAY DIFFRACTION / Resolution: 2.56 Å PDB-9i4e: Structure of anti-EV71 human monoclonal antibody 34-1-6D Fab Method: X-RAY DIFFRACTION / Resolution: 1.63 Å
Chemicals	ChemComp-BTB: 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH bufferYM ChemComp-EDO: 1,2-ETHANEDIOL ChemComp-HOH: WATER ChemComp-SO4: SULFATE ION ChemComp-GOL: GLYCEROL ChemComp-CL: Unknown entry ChemComp-ACT: ACETATE ION ChemComp-SPH*: SPHINGOSINE
Source	Enterovirus A71 homo sapiens (human) chlorocebus aethiops (grivet monkey) human enterovirus 71
Keywords	ANTIVIRAL PROTEIN / EV71 / antibody / 16-2-8C / Fab / 16-2-12D / 16-3-4D / 16-3-3C / 16-2-2D / 16-2-9D / 16-3-10B / 17-2-12A / 17-1-12A / 17-2-2B / 34-1-6D / VIRUS / Hand-foot-and-mouth disease / HFMD / enterovirus 71 (EV-A71) / Human antibody / 17-1-2B / 16-2-11B