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TitleInsights into the structure and evolution of the human SAGA complex by affinity-ligand purification.
Journal, issue, pagesSci Adv, Vol. 12, Issue 12, Page eaec8104, Year 2026
Publish dateMar 20, 2026
AuthorsMylène Damilot / Thomas Schoeps / Laszlo Tora / Patrick Schultz / Luc Lebeau / Gabor Papai / Adam Ben-Shem /
PubMed AbstractHuman SAGA is a 20-subunit transcriptional coactivator. Compared with yeast, metazoan SAGA uniquely incorporates a 150-kDa splicing-factor module (SPL), also present in U2 small nuclear ...Human SAGA is a 20-subunit transcriptional coactivator. Compared with yeast, metazoan SAGA uniquely incorporates a 150-kDa splicing-factor module (SPL), also present in U2 small nuclear ribonucleoprotein (U2snRNP). Metazoan gene duplication further specialized shared TFIID/SAGA subunits into SAGA-specific paralogs (TAF5L and TAF6L), but the functional consequences of this divergence are unknown. We report the structure of endogenous human SAGA purified via an affinity ligand from cells that were not disturbed by any genomic engineering tools. Our work reveals the high-resolution structure of SPL and the TAF6L HEAT repeat domain that provides the SPL with a docking surface. We elucidate how SPL and the HEAT repeats are incorporated into SAGA. We identify major structural differences between TAF6L/TAF5L and their canonical paralogs that enable SPL accommodation. SPL engages SAGA through a substantially smaller interface than in U2snRNP, despite sharing a deeply inserted helical motif. The seemingly weaker interaction of SPL with SAGA raises the possibility that SAGA relays this module to the splicing machinery.
External linksSci Adv / PubMed:41849588 / PubMed Central
MethodsEM (single particle)
Resolution2.38 - 3.24 Å
Structure data

EMDB-53895: Human SAGA core Domain
Method: EM (single particle) / Resolution: 2.58 Å

EMDB-53896: Focus refined map of TRRAP in human SAGA
Method: EM (single particle) / Resolution: 2.38 Å

EMDB-53932: Focus refined map of human SAGA TRRAP end
Method: EM (single particle) / Resolution: 3.24 Å

EMDB-53934: Focus refined map of Human SAGA SF3B module
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-53935: Human SAGA consensus map
Method: EM (single particle) / Resolution: 2.41 Å

53937

9rdk

EMDB-53937, PDB-9rdk:
Cryo-EM structure of the human SAGA co-activator complex
Method: EM (single particle) / Resolution: 2.41 Å

Source
  • homo sapiens (human)
KeywordsTRANSCRIPTION / Transcription co-activator / Lysine acetyltransferase / complex

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