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TitleStructural insights into the antibacterial function of the Pseudomonas putida effector Tke5.
Journal, issue, pagesEMBO J, Year 2026
Publish dateJan 12, 2026
AuthorsCarmen Velázquez / Maialen Zabala-Zearreta / Carmen Paredes / Cristina Civantos / Jon Altuna-Alvarez / Patricia Bernal / David Albesa-Jové /
PubMed AbstractPseudomonas putida is a plant-beneficial rhizobacterium that encodes multiple type-VI secretion systems (T6SS) to outcompete phytopathogens in the rhizosphere. Among its antibacterial effectors, Tke5 ...Pseudomonas putida is a plant-beneficial rhizobacterium that encodes multiple type-VI secretion systems (T6SS) to outcompete phytopathogens in the rhizosphere. Among its antibacterial effectors, Tke5 (a member of the BTH_I2691 protein family) is a potent pore-forming toxin that disrupts ion homeostasis without causing considerable membrane damage. Tke5 harbours an N-terminal MIX domain, which is required for T6SS-dependent secretion in other systems. Many MIX domain-containing effectors require T6SS adaptor proteins (Tap) for secretion, but their molecular mechanisms of adaptor-effector binding remain elusive. Here, we report the 2.8 Å cryo-EM structure of the Tap3-Tke5 complex of P. putida strain KT2440, providing structural and functional insights into how effector Tke5 is recruited by its cognate adaptor protein Tap3. Functional dissection shows that the α-helical region of Tke5 is sufficient to kill intoxicated bacteria, while its β-rich region likely contributes to target membrane specificity. These findings delineate a mechanism of BTH_I2691 proteins for Tap recruitment and toxin activity, contributing to our understanding of a widespread yet understudied toxin family.
External linksEMBO J / PubMed:41526723
MethodsEM (single particle)
Resolution2.77 Å
Structure data

53820

9r8g

EMDB-53820, PDB-9r8g:
Pseudomonas putida Pore-Forming Toxin Tke5 in complex with its cognate Type VI adaptor protein Tap3
Method: EM (single particle) / Resolution: 2.77 Å

Source
  • pseudomonas putida kt2440 (bacteria)
KeywordsTOXIN / Pseudomonas putida T6SS effector / Pore-Forming Toxin / MIX family domain / adaptor protein

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