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Structure paper

TitleStructural basis for transcriptional regulation by the cell division regulator MraZ in Mycoplasma genitalium.
Journal, issue, pagesNat Commun, Vol. 17, Issue 1, Year 2026
Publish dateJan 29, 2026
AuthorsLucía Sánchez-Alba / Nathalia Varejão / Alexandre Durand / Javier García-Pardo / Maria Carreras-Caballé / Virginia Amador / Jaume Pinyol / David Reverter /
PubMed AbstractCell division is a central process in all living organisms and requires the coordinated action of many proteins and regulatory elements. In most bacteria, the division and cell wall (dcw) gene ...Cell division is a central process in all living organisms and requires the coordinated action of many proteins and regulatory elements. In most bacteria, the division and cell wall (dcw) gene cluster is regulated by the first gene of the dcw operon, mraZ, a highly conserved DNA-binding transcriptional regulator. Here we report the structural basis of MraZ transcriptional regulation by the resolution of three different cryo-EM structures of MraZ in complex with the upstream promoter region of the dcw cluster from Mycoplasma genitalium at 3.36, 3.57 and 3.87 Å resolution. The structures reveal the specific interactions between MraZ DNA-binding motif and nucleobases of the binding boxes, which induces distortion in the MraZ octamer to enable the interaction with the four repetitive binding boxes of the promoter DNA. The "cradle-like" DNA-binding motif of MraZ exposes three highly conserved basic residues, Lys13, Arg15 and Arg86, which are essential for binding to the consensus sequence of its cognate promoter. Ultimately, the mechanism behind MraZ's DNA binding and regulation of the dcw operon could be translated to other species, working as a general mechanism for the regulation of dcw gene cluster in bacteria.
External linksNat Commun / PubMed:41611686 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.96 - 3.87 Å
Structure data

53569

9r4j

EMDB-53569, PDB-9r4j:
CryoEM structure of MraZ in complex with its promoter from Mycoplasma genitalium
Method: EM (single particle) / Resolution: 3.36 Å

55332

9sx6

EMDB-55332, PDB-9sx6:
CryoEM structure of the octamer MraZ in complex with 1 box promoter from Mycoplasma genitalium
Method: EM (single particle) / Resolution: 3.57 Å

55361

9sz7

EMDB-55361, PDB-9sz7:
CryoEM structure of MraZ in complex with 4 box promoter from Mycoplasma genitalium
Method: EM (single particle) / Resolution: 3.87 Å

PDB-9qlg:
Crystal structure of the transcription factor MraZ from Mycoplasma genitalium
Method: X-RAY DIFFRACTION / Resolution: 1.96 Å

PDB-9qlr:
Nonamer crystal structure of the transcription factor MraZ from Mycoplasma genitalium
Method: X-RAY DIFFRACTION / Resolution: 3.85 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • mycoplasmoides genitalium (bacteria)
KeywordsTRANSCRIPTION / Transcription factor / octameric / nonamer / DNA BINDING PROTEIN / MraZ

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