Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of lipid-linked galactan export by the mycobacterial ABC transporter Wzm-Wzt.
Journal, issue, pages	Nat Commun, Vol. 17, Issue 1, Year 2026
Publish date	Mar 16, 2026
Authors	Alisa A Garaeva / Viktória Fabianová / Karin Savková / Stanislav Huszár / Xiaochao Xue / Todd L Lowary / Katarína Mikušová / Markus A Seeger /
PubMed Abstract	Mycobacteria, including Mycobacterium tuberculosis, possess a unique cell envelope containing arabinogalactan, a heteropolysaccharide critical for cell wall integrity and target of several ...Mycobacteria, including Mycobacterium tuberculosis, possess a unique cell envelope containing arabinogalactan, a heteropolysaccharide critical for cell wall integrity and target of several tuberculosis drugs. The cytosolic precursor of arabinogalactan, lipid-linked galactan (LLG), is translocated across the plasma membrane by the essential ABC transporter Wzm-Wzt through a molecular mechanism that is poorly understood. Here, we present a series of cryo-EM structures of Wzm-Wzt from Mycobacterium abscessus, representing different conformations of the transport cycle. Conserved residues lining the proposed LLG translocation pathway were investigated by three orthologous functional assays, revealing that the cytosolic gate helix (GH) plays a key functional role in polysaccharide transport. Our data suggests that the hydrophobic polyprenyl-moiety is translocated first, followed by the galactan-polysaccharide, which requires Wzm-Wzt to open a continuous channel through which the sugar chain is ratcheted at the expense of ATP hydrolysis. Our results provide a rational basis for the development of drugs that inhibit mycobacterial cell wall biosynthesis.
External links	Nat Commun / PubMed:41839883 / PubMed Central
Methods	EM (single particle)
Resolution	3.3 - 4.04 Å
Structure data	53123 9qfx EMDB-53123, PDB-9qfx: Nucleotide-free wild type Wzm-Wzt from Mycobacterium abscessus in LMNG Method: EM (single particle) / Resolution: 3.45 Å 53148 9qgu EMDB-53148, PDB-9qgu: ADP-bound wild type Wzm-Wzt from Mycobacterium abscessus in LMNG Method: EM (single particle) / Resolution: 3.69 Å 53152 9qh1 EMDB-53152, PDB-9qh1: ATP-bound wild type Wzm-Wzt from Mycobacterium abscessus in LMNG Method: EM (single particle) / Resolution: 3.44 Å 53171 9qhj EMDB-53171, PDB-9qhj: ATP-bound E178Q Wzm-Wzt from Mycobacterium abscessus in LMNG Method: EM (single particle) / Resolution: 3.4 Å 53172 9qhk EMDB-53172, PDB-9qhk: Nucleotide-free wild type Wzm-Wzt from Mycobacterium abscessus in nanodiscs Method: EM (single particle) / Resolution: 3.57 Å 53180 9qhv EMDB-53180, PDB-9qhv: ADP-bound wild type Wzm-Wzt from Mycobacterium abscessus in nanodiscs Method: EM (single particle) / Resolution: 4.04 Å 53181 9qhw EMDB-53181, PDB-9qhw: ATP-bound wild type Wzm-Wzt from Mycobacterium abscessus in nanodiscs Method: EM (single particle) / Resolution: 3.3 Å 53182 9qhx EMDB-53182, PDB-9qhx: ATP-bound E178Q Wzm-Wzt from Mycobacterium abscessus in nanodiscs Method: EM (single particle) / Resolution: 3.47 Å
Chemicals	ChemComp-AV0: Lauryl Maltose Neopentyl Glycol ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / phospholipidYM ChemComp, No image ChemComp-UNL*: Unknown ligand
Source	mycobacteroides abscessus (bacteria)
Keywords	MEMBRANE PROTEIN / ABC transporter Type V ABC transporter Arabinogalactan precursor Lipid-linked galactan exporter