EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of lymphostatin, a large multi-functional virulence factor of pathogenic Escherichia coli.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 5389, Year 2025
掲載日	2025年6月25日
著者	Matthias Griessmann / Tim Rasmussen / Vanessa J Flegler / Christian Kraft / Ronja Schneider / Max Hateley / Lukas Spantzel / Mark P Stevens / Michael Börsch / Bettina Böttcher /
PubMed 要旨	Lymphostatin is a key virulence factor of enteropathogenic and enterohaemorrhagic Escherichia coli, playing roles in bacterial colonisation of the gut and in the inhibition of lymphocyte ...Lymphostatin is a key virulence factor of enteropathogenic and enterohaemorrhagic Escherichia coli, playing roles in bacterial colonisation of the gut and in the inhibition of lymphocyte proliferation and proinflammatory responses. The protein's glycosyltransferase and cysteine protease motifs are required for activity against lymphocytes, but high-resolution structural information has proven elusive. Here, we describe the structure of lymphostatin from enteropathogenic E. coli O127:H6, determined by electron cryo-microscopy at different pH values. We observe three conformations of a highly complex molecule with two glycosyltransferase domains, one PaToxP-like protease domain, an ADP-ribosyltransferase domain, a vertex domain and a delivery domain. Long linkers hold these domains together and occlude the catalytic sites of the N-terminal glycosyltransferase and protease domains. Lymphostatin binds to bovine T-lymphocytes and HEK-293T cells, forming clusters at the plasma membrane that are internalized. With six distinct domains, lymphostatin can be regarded as a multitool of pathogenic Escherichia coli, enabling complex interactions with host cells.
リンク	Nat Commun / PubMed:40562750 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 4.4 Å
構造データ	EMDB-19982: Lymphostatin, conformation 1, N-terminal part 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-19983: Lymphostatin, conformation 1, local refinement C-terminal part 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-19984: Lymphostatin, conformation 2, N-terminal part 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-19985: Lymphostatin, conformation 2 , local refinement C-terminal part 手法: EM (単粒子) / 解像度: 3.8 Å 19987 9euv EMDB-19987, PDB-9euv: Lymphostatin, conformation 1 (composite structure) 手法: EM (単粒子) / 解像度: 3.0 Å 19988 9euw EMDB-19988, PDB-9euw: Lymphostatin, conformation 2 (composite structure) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-52985: Lymphostatin Conformation II, Hepes buffer pH8 - consensus map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-52986: Lymphostatin -Conformation II - Local-Refinement GT-domains 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-52987: Lymphostatin - Conformation II - pH 8, Hepes, focussed on center 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-52988: Lymphostatin - Conformation II - pH8 Hepes, focussed on delivery 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-52989: Lymphostatin - Conformation II - pH 8 Hepes - focussed on C-term 手法: EM (単粒子) / 解像度: 4.2 Å 52990 9qb8 EMDB-52990, PDB-9qb8: Lymphostatin - Conformation II - pH 8 Hepes 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-52991: Lymphostatin - conformation III - pH 8 focussed on delivery domain 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-52992: Lymphostatin - Conformation III - pH 8 focussed on centre 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-52993: Lymphostatin - Conformation III - pH 8 - focussed on C-term 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-52994: Lymphostatin - conformation III - pH 8 focussed on N-term 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-52995: Lymphostatin - Conformation III - pH 8 - consensus map 手法: EM (単粒子) / 解像度: 3.3 Å 52996 9qbb EMDB-52996, PDB-9qbb: Lymphostatin - Conformation III - pH 8 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-53164: Lymphostatin - pH8 phosphate buffer - focussed on C-term 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-53165: Lymphostatin- pH 8.0 phosphate buffer - focussed on delivery domain S1 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-53166: Lymphostatin - pH 8 phosphate buffer - focussed on centre 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-53167: Lymphostatin - pH 8.0 phosphate - focussed on GT-domain 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-53168: Lymphostatin at pH 8 in Phosphate buffer - Consensus Map 手法: EM (単粒子) / 解像度: 3.2 Å 53169 9qhh EMDB-53169, PDB-9qhh: Lymphostatin - pH 8 - phosphate buffer 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-53286: Lymphostatin - Conformation I - pH 6.5 phosphate buffer 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-53287: Lymphostatin - conformation II - pH 6.5 phosphate buffer 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-MN: Unknown entry
由来	escherichia coli o127:h6 str. e2348/69 (大腸菌) escherichia coli o127:h6 (大腸菌) Escherichia coli O2:H6 (大腸菌) Escherichia coli (大腸菌)
キーワード	TOXIN / bacterial virulence factor / glycosyltransferase / protease / Virulence Factor Lymphostatin LifA Conformation II / Virulence Factor Lymphostatin LifA Conformation III / Lymphostatin virulence factor A/E bacteria Toxin-like