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-Structure paper
| タイトル | Segmented filamentous bacteria undergo a structural transition at their adhesive tip during unicellular to filament development. |
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| ジャーナル・号・ページ | Nat Commun, Vol. 17, Issue 1, Page 222, Year 2025 |
| 掲載日 | 2025年12月17日 |
 著者 | Ana Raquel Cruz / Benedikt H Wimmer / Teck Hui Teo / Gérard Pehau-Arnaudet / Jean-Marie Winter / Anastasia D Gazi / Pierre Lafaye / Sébastien Brier / Agnès Legrand / Anna Dubrovsky-Gaupp / Marion Bérard / Gabriel Aymé / Anna Sartori-Rupp / Ohad Medalia / Pamela Schnupf /    |
| PubMed 要旨 | Segmented filamentous bacteria (SFB) are intestinal commensals that promote immune system development and pathogen protection through intimate attachment to the ileal epithelium. Attachment occurs ...Segmented filamentous bacteria (SFB) are intestinal commensals that promote immune system development and pathogen protection through intimate attachment to the ileal epithelium. Attachment occurs via the tip of unicellular teardrop-shaped SFB, called intracellular offsprings (IOs), before outgrowth into filaments. To characterize this critical stage of the SFB life cycle, we imaged SFB using cryo-electron microscopy and tomography. IOs were surrounded by a repetitive surface (S)-layer that became replaced by disordered hair-like structures specifically at the IO tip. During outgrowth into filaments, the S-layer was exchanged for a morphologically distinct repetitive hair-like layer. The bacterial structures and morphological transition were conserved across SFB from mouse and rat origin, while growth of mouse-SFB under non-attachment conditions in a heterologous host affected the SFB tip length and relative proportion of the tip stages. Moreover, a major Th17 and B cell antigen, while being a ubiquitous cell wall-associated protein, was immunologically accessible only at the filament tip, underscoring the unique properties of the tip structure. This study identifies an IO-specific S-layer and reveals a conserved developmental transition of the SFB tip surface including the transient appearance of structures consistent in location and timing with being involved in host cell attachment. |
 リンク | Nat Commun / PubMed:41407704 / PubMed Central |
| 手法 | EM (トモグラフィー) |
| 構造データ |  EMDB-52655: Cryo-electron tomogram of the S-layer coated tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing flagella and intracellular vesicles) grown in mice  EMDB-52667: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing a chemosensory array) grown in mice  EMDB-52668: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing intracellular filaments) grown in mice  EMDB-52669: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing intracellular plate-like structures) grown in mice  EMDB-52670: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing intracellular vesicles) grown in mice and of undecorated extracellular vesicle  EMDB-52671: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring) grown in mice and of decorated extracellular vesicle  EMDB-52673: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing intracellular plate-like structures and a chemosensory array) grown in mice  EMDB-52674: Cryo-electron tomogram of the cell body of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing intracellular filaments) grown in mice  EMDB-52675: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring) grown in mice and of undecorated extracellular vesicles  EMDB-52676: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 3 intracellular offspring) grown in mice  EMDB-52677: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 4 filament) grown in mice  EMDB-52678: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 5 filament) grown in mice  EMDB-52679: Cryo-electron tomogram of the hair-like layer coated distal end of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring) grown in mice  EMDB-52680: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 3 intracellular offspring) grown in mice  EMDB-52682: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 5 intracellular offspring) grown in mice  EMDB-52683: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (transitional stage 4 intracellular offspring) grown in mice  EMDB-52684: Cryo-electron tomogram of the S-layer coated tip of Candidatus Arthromitus sp. SFB-rat-Yit (intracellular offspring containing intracellular vesicles) grown in rats  EMDB-52685: Cryo-electron tomogram of the S-layer coated tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 1 intracellular offspring) grown in mice  EMDB-52687: Cryo-electron tomogram of the S-layer coated tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing flagella) grown in mice  EMDB-52688: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (stage 3 intracellular offspring) grown in rats  EMDB-52689: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (intracellular offspring containing flagella and intracellular filaments) grown in rats and of decorated extracellular vesicles  EMDB-52690: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (unidentified stage intracellular offspring) grown in rats  EMDB-52691: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (intracellular offspring containing intracellular plate-like structures) grown in rats  EMDB-52692: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (intracellular offspring containing flagella and a chemosensory array) grown in rats  EMDB-52693: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (intracellular offspring containing intracellular filaments and vesicles) grown in rats and of extracellular vesicles  EMDB-52694: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (intracellular offspring containing intracellular plate-like structures) grown in rats  EMDB-52695: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (stage 1 intracellular offspring) grown in rats  EMDB-52696: Cryo-electron tomogram of the S-layer coated distal end of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring) grown in mice  EMDB-52697: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (unidentified stage intracellular offspring) grown in mice  EMDB-52698: Cryo-electron tomogram of the S-layer coated distal end of Candidatus Arthromitus sp. SFB-rat-Yit (intracellular offspring) grown in rats  EMDB-52699: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (stage 5 filament) grown in rats  EMDB-52700: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 1 intracellular offspring) grown in rats  EMDB-52701: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 2 intracellular offspring) grown in rats  EMDB-52702: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 3 intracellular offspring) grown in rats  EMDB-52703: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 5 intracellular offspring) grown in rats  EMDB-52856: Cryo-electron tomogram of the distal end of Candidatus Arthromitus sp. SFB-rat-Yit (unidentified stage intracellular offspring) grown in rats  EMDB-54603: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (transitional stage 3 intracellular offspring) grown in rats  EMDB-54605: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (intracellular offspring containing short and long tracks) grown in mice  EMDB-54607: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-rat-Yit (stage 5 filament) grown in rats  EMDB-54608: Cryo-electron tomogram of the tip of Candidatus Arthromitus sp. SFB-mouse-NL (stage 4 filament) grown in mice |
| 由来 |
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Candidatus Arthromitus sp. SFB-mouse-NL (バクテリア)