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TitleStructural and mechanistic insights into the constitutive Themis-Grb2 complex in T cell signalling.
Journal, issue, pagesNat Commun, Year 2026
Publish dateMay 20, 2026
AuthorsDanielle M Clancy / Alba Sanz-Sanjuan / Elisabeth Gilis / Peter Tougaard / Imke Velghe / Yana Van Droogenbroeck / Jan Felix / Yehudi Bloch / Álvaro Furones Cuadrado / Romain Merceron / Stephan Schenck / Peter Vandenabeele / Janine D Brunner / Tom Taghon / Dirk Elewaut / Savvas N Savvides /
PubMed AbstractThymocyte selection is essential for establishing the T cell repertoire, maintaining self-tolerance and preventing autoimmunity. Themis, the archetypal member of a metazoan protein family defined by ...Thymocyte selection is essential for establishing the T cell repertoire, maintaining self-tolerance and preventing autoimmunity. Themis, the archetypal member of a metazoan protein family defined by CABIT domains, centrally regulates this process by integrating T cell receptor (TCR) signalling. Themis has been proposed to constitutively partner with the multifunctional adaptor Grb2, yet the structural and mechanistic basis of this assembly has remained enigmatic. Here, we use Cryo-EM to reveal how the tandem CABIT domains and proline-rich sequence of Themis cooperatively engulf the C-terminal SH3 domain of Grb2, while the unbound domains of Grb2 remain poised to recruit additional binding partners. Furthermore, we uncover inherent interdomain flexibility in unbound Themis that resolves upon Grb2 binding. Structure-guided mutations abrogate the Themis-Grb2 interaction and fail to regulate the tyrosine phosphatase SHP-1 after TCR stimulation, recapitulating the phenotype of Themis-deficient cells. Our findings define the Themis-Grb2 complex as a dynamic structural hub in T cell signalling.
External linksNat Commun / PubMed:42161968
MethodsEM (single particle)
Resolution3.2 - 7.0 Å
Structure data

52603

9i3p

EMDB-52603, PDB-9i3p:
CryoEM structure of the Themis:Grb2 complex with bound ProMacrobody 256
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-52604: 3D reconstruction of Themis:Grb2:ProMacrobody256 after 3D classification (Class 1)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-52605: 3D reconstruction of Themis:Grb2:ProMacrobody256 after 3D classification (Class 2)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-52606: 3D reconstruction of Themis:Grb2:ProMacrobody256 after 3D classification (Class 3)
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-52607: 3D reconstruction of Themis:Grb2:ProMacrobody256 after 3D classification (Class 4)
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-52608: CryoEM map of Themis bound to ProMacrobody 256
Method: EM (single particle) / Resolution: 5.8 Å

EMDB-52609: 3D reconstruction of Themis:ProMacrobody256 after 3D classification (Class 1)
Method: EM (single particle) / Resolution: 6.3 Å

EMDB-52610: 3D reconstruction of Themis:ProMacrobody256 after 3D classification (Class 2)
Method: EM (single particle) / Resolution: 7.0 Å

52787

9iaz

EMDB-52787, PDB-9iaz:
CryoEM structure of the Themis:Grb2 complex with bound ProMacrobody 256, local refinement
Method: EM (single particle) / Resolution: 3.2 Å

Source
  • homo sapiens (human)
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsSIGNALING PROTEIN / adapter protein / CABIT / TCR / T cell development

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