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TitleStructural Basis for Postfusion-Specific Binding to the Respiratory Syncytial Virus F Protein by the Canonical Antigenic Site I Antibody 131-2a.
Journal, issue, pagesACS Infect Dis, Vol. 11, Issue 8, Page 2357-2366, Year 2025
Publish dateAug 8, 2025
AuthorsWeiwei Peng / Marta Šiborová / Xuesheng Wu / Wenjuan Du / Douwe Schulte / Matti F Pronker / Cornelis A M de Haan / Joost Snijder /
PubMed AbstractThe respiratory syncytial virus (RSV) fusion (F) protein is a major target of antiviral antibodies following natural infection or vaccination and is responsible for mediating fusion between the viral ...The respiratory syncytial virus (RSV) fusion (F) protein is a major target of antiviral antibodies following natural infection or vaccination and is responsible for mediating fusion between the viral envelope and the host membrane. The fusion process is driven by a large-scale conformational change in F, switching irreversibly from the metastable prefusion state to the stable postfusion conformation. Previous research has identified six distinct antigenic sites in RSV-F, termed sites Ø, I, II, III, IV, and V. Of these, only antigenic site I is fully specific to the postfusion conformation of F. A monoclonal antibody 131-2a that specifically targets postfusion F has been widely used as a research tool to probe for postfusion F and to define antigenic site I in serological studies, yet its sequence and precise epitope have remained unknown. Here, we use mass spectrometry-based sequencing of 131-2a to reverse engineer a recombinant product and study the epitope to define antigenic site I with molecular detail, revealing the structural basis for the antibody's specificity toward postfusion RSV-F.
External linksACS Infect Dis / PubMed:40693554 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.3 Å
Structure data

52444

9hvw

EMDB-52444, PDB-9hvw:
Respiratory Syncytial Virus Fusion protein in the postfusion conformation in complex with monoclonal antibody 131-2a Fab
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-52446: RSV-F postfusion trimer with no 131-2a bound
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-52447: RSV-F postfusion trimer with one 131-2a Fab bound
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-52448: RSV-F postfusion trimer with two 131-2a Fabs bound
Method: EM (single particle) / Resolution: 3.3 Å

Source
  • human respiratory syncytial virus
  • mus musculus (house mouse)
KeywordsVIRAL PROTEIN / antigenic site I

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