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TitleStructure of the F-tractin-F-actin complex.
Journal, issue, pagesJ Cell Biol, Vol. 224, Issue 4, Year 2025
Publish dateApr 7, 2025
AuthorsDmitry Shatskiy / Athul Sivan / Roland Wedlich-Söldner / Alexander Belyy /
PubMed AbstractF-tractin is a peptide widely used to visualize the actin cytoskeleton in live eukaryotic cells but has been reported to impair cell migration and induce actin bundling at high expression levels. To ...F-tractin is a peptide widely used to visualize the actin cytoskeleton in live eukaryotic cells but has been reported to impair cell migration and induce actin bundling at high expression levels. To elucidate these effects, we determined the cryo-EM structure of the F-tractin-F-actin complex, revealing that F-tractin consists of a flexible N-terminal region and an amphipathic C-terminal helix. The N-terminal part is dispensable for F-actin binding but responsible for the bundling effect. Based on these insights, we developed an optimized F-tractin, which eliminates the N-terminal region and minimizes bundling while retaining strong actin labeling. The C-terminal helix interacts with a hydrophobic pocket formed by two neighboring actin subunits, an interaction region shared by many actin-binding polypeptides, including the popular actin-binding probe Lifeact. Thus, rather than contrasting F-tractin and Lifeact, our data indicate that these peptides have analogous modes of interaction with F-actin. Our study dissects the structural elements of F-tractin and provides a foundation for developing future actin probes.
External linksJ Cell Biol / PubMed:39928047 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.4 Å
Structure data

51496

9gob

EMDB-51496, PDB-9gob:
Structure of the F-tractin-F-actin complex
Method: EM (single particle) / Resolution: 3.2 Å

52289

9hm9

EMDB-52289, PDB-9hm9:
Structure of the optimized F-tractin in complex with F-actin
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • oryctolagus cuniculus (rabbit)
  • rattus norvegicus (Norway rat)
KeywordsCYTOSOLIC PROTEIN / Actin / F-tractin

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