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TitleThe kidney slit diaphragm resembles a fishnet.
Journal, issue, pagesKidney Int, Vol. 108, Issue 6, Page 1045-1056, Year 2025
Publish dateSep 24, 2025
AuthorsAlexandra N Birtasu / Konstantin Wieland / Utz H Ermel / Serena M Arghittu / Sina Manger / Maciej K Kocylowski / Bernd Fakler / Margot P Scheffer / Roberto Covino / Florian Grahammer / Achilleas S Frangakis /
PubMed AbstractINTRODUCTION: An essential component of the kidney filtration barrier, the slit diaphragm, is a specialized cell-cell junction composed of the cell adhesion molecules Nephrin and Neph1.METHODS: Here, ...INTRODUCTION: An essential component of the kidney filtration barrier, the slit diaphragm, is a specialized cell-cell junction composed of the cell adhesion molecules Nephrin and Neph1.
METHODS: Here, we use cryo-electron tomography of vitreous lamellae from high pressure frozen wild-type C57BL/6J native mouse glomeruli.
RESULTS: The cryo-electron tomograms show that the slit diaphragm resembles a fishnet-like architecture. After sub-tomogram averaging and molecular dynamics flexible fitting of Nephrin and Neph1, the resulting molecular model of the slit diaphragm reveals a criss-cross arrangement of these cell adhesion molecules with multiple homo- and hetero-philic interactions. This renders the slit diaphragm as a single- or a multi-layer fishnet, quasi-crystalline, sheet-like molecular polymer that can provide both stability and flexibility to compensate for mechanical forces.
CONCLUSIONS: The molecular architecture of the murine slit diaphragm provides a mechanistic framework for comprehending kidney cell-cell junctions with immediate implications in disease.
External linksKidney Int / PubMed:41005566
MethodsEM (subtomogram averaging)
Resolution36.0 Å
Structure data

EMDB-52069: The molecular architecture of the murine kidney slit diaphragm revealed by cryo-electron tomography
Method: EM (subtomogram averaging) / Resolution: 36.0 Å

Source
  • Mus musculus (house mouse)

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