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TitleStructural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 402, Year 2025
Publish dateJan 5, 2025
AuthorsAnjun Zheng / Bram J A Vermeulen / Martin Würtz / Annett Neuner / Nicole Lübbehusen / Matthias P Mayer / Elmar Schiebel / Stefan Pfeffer /
PubMed AbstractThe γ-tubulin ring complex (γ-TuRC) is a structural template for controlled nucleation of microtubules from α/β-tubulin heterodimers. At the cytoplasmic side of the yeast spindle pole body, the ...The γ-tubulin ring complex (γ-TuRC) is a structural template for controlled nucleation of microtubules from α/β-tubulin heterodimers. At the cytoplasmic side of the yeast spindle pole body, the CM1-containing receptor protein Spc72 promotes γ-TuRC assembly from seven γ-tubulin small complexes (γ-TuSCs) and recruits the microtubule polymerase Stu2, yet their molecular interplay remains unclear. Here, we determine the cryo-EM structure of the Candida albicans cytoplasmic nucleation unit at 3.6 Å resolution, revealing how the γ-TuRC is assembled and conformationally primed for microtubule nucleation by the dimerised Spc72 CM1 motif. Two coiled-coil regions of Spc72 interact with the conserved C-terminal α-helix of Stu2 and thereby position the α/β-tubulin-binding TOG domains of Stu2 in the vicinity of the microtubule assembly site. Collectively, we reveal the function of CM1 motifs in γ-TuSC oligomerisation and the recruitment of microtubule polymerases to the γ-TuRC.
External linksNat Commun / PubMed:39757296 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 8.2 Å
Structure data

51971

9h9q

EMDB-51971, PDB-9h9q:
Candida albicans gamma-tubulin small complex within ring-like higher oligomer in complex with Spc72 CM1
Method: EM (single particle) / Resolution: 3.6 Å

51972

9h9r

EMDB-51972, PDB-9h9r:
Full gamma-tubulin ring complex composed of the Candida albicans gamma-tubulin small complex in complex with Spc72 CM1
Method: EM (single particle) / Resolution: 8.2 Å

PDB-9h9p:
Spokes 12 and 13 of the human gamma-tubulin ring complex in complex with CDK5RAP2 and docked MZT2/GCP2-NHD module
Method: ELECTRON MICROSCOPY / Resolution: 4.5 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • candida albicans (yeast)
  • homo sapiens (human)
KeywordsCELL CYCLE / cytoskeleton / microtubule / microtubule nucleation / complex / template / cap / gamma-tubulin / gamma-tubulin ring complex / CDK5RAP2 / Homo / sapiens / GCP6 / GCP2 / gamma-tubulin complex protein / MZT2 / Mozart / Mozart2 / module / CM1 / CM1 motif / CEP215 / gamma-tubulin small complex / Spc72 / GCP3 / Spc97 / Spc98 / Candida / albicans / gamma-TuRC / gamma-TuSC / yeast / fungi

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