Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structures of transcription complexes reveal how ToxR and TcpP recruit the RNA polymerase and activate virulence genes.
Journal, issue, pages	Sci Adv, Vol. 12, Issue 3, Page eadx9680, Year 2026
Publish date	Jan 16, 2026
Authors	Adrià Alcaide-Jiménez / Albert Canals / Florence Baudin / Cristina Machón / Montserrat Fàbrega-Ferrer / Olga Bantysh / Rosa Pérez-Luque / Brice Murciano / Ali A Mohammad / Michael J Rowse / Joseph M Ferracciolo / Eric S Krukonis / Christoph W Müller / Miquel Coll /
PubMed Abstract	Activation of virulence in , the etiological agent of cholera disease, is mediated by two transmembrane one-component signal-transduction proteins, ToxR and TcpP, which are also transcription factors. ...Activation of virulence in , the etiological agent of cholera disease, is mediated by two transmembrane one-component signal-transduction proteins, ToxR and TcpP, which are also transcription factors. Using cryo-electron microscopy, we have solved five structures of the and transcription activation complexes, including the RNA polymerase (RNAP) holoenzyme, promoter DNAs, transcribed RNA, and their corresponding transcription factors, ToxR or TcpP and ToxR-TcpP, respectively. Activation is achieved through the interaction of ToxR or TcpP with the α-C-terminal repeat domain of RNAP where a single residue of the activator, a phenylalanine, appears to be the most critical contact, as confirmed by mutagenesis. No interactions of the transcription factors were observed with other subunits of the RNAP, i.e., the σ subunit as it occurs in the structurally related PhoB family of two-component transcription factors. The structures, and their comparison with our previously solved DNA promoter-ToxR x-ray structures, unveil the molecular mechanism of cholera virulence gene activation.
External links	Sci Adv / PubMed:41533787 / PubMed Central
Methods	EM (single particle)
Resolution	2.6 - 7.2 Å
Structure data	51273 9gdo EMDB-51273, PDB-9gdo: Cryo-EM structure of Vibrio cholerae RNA polymerase holoenzyme bound to an ompU promoter DNA fragment Method: EM (single particle) / Resolution: 3.0 Å 51274 9gdp EMDB-51274, PDB-9gdp: Cryo-EM structure of Vibrio cholerae RNA polymerase holoenzyme bound to an ompU promoter DNA fragment and 5-mer RNA Method: EM (single particle) / Resolution: 2.6 Å 51275 9gdq EMDB-51275, PDB-9gdq: Cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and ompU promoter DNA Method: EM (single particle) / Resolution: 3.1 Å 51276 9gdr EMDB-51276, PDB-9gdr: Cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 2.7 Å 51277 9gds EMDB-51277, PDB-9gds: Cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 2.9 Å EMDB-51278: Cryo-EM structure of Vibrio cholerae RNA polymerase dimer with ToxR and TcpP transcription factors and a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 7.2 Å EMDB-51774: Consensus map of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and bound to an ompU promoter DNA fragment Method: EM (single particle) / Resolution: 3.1 Å EMDB-51775: Focused map #1 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and bound to an ompU promoter DNA fragment Method: EM (single particle) / Resolution: 3.1 Å EMDB-51776: Focused map #2 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and bound to an ompU promoter DNA fragment Method: EM (single particle) / Resolution: 3.8 Å EMDB-51948: Consensus map of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and bound to a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 2.7 Å EMDB-51949: Focused map #1 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and bound to a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 2.7 Å EMDB-51950: Focused map #2 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with TcpP transcription factor and bound to a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 2.7 Å EMDB-51955: Consensus map of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and bound to a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 2.9 Å EMDB-51956: Focused map #1 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and bound to a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 2.9 Å EMDB-51958: Focused map #2 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR and TcpP transcription factors and bound to a toxT promoter DNA fragment Method: EM (single particle) / Resolution: 2.9 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry
Source	vibrio cholerae o395 (bacteria) vibrio cholerae o1 biovar el tor str. n16961 (bacteria)
Keywords	TRANSCRIPTION / RNA polymerase / Open promoter complex / ompU / Vibrio cholerae / Transcription Activation Complex / toxT / TcpP / ToxR