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TitleUnwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx.
Journal, issue, pagesAdv Sci (Weinh), Vol. 12, Issue 16, Page e2413355, Year 2025
Publish dateMar 5, 2025
AuthorsLara M Hoepfner / Adrian P Nievergelt / Fabrizio Matrino / Martin Scholz / Helen E Foster / Jonathan Rodenfels / Alexander von Appen / Michael Hippler / Gaia Pigino /
PubMed AbstractThe glycocalyx, a highly heterogeneous glycoprotein layer of cilia regulates adhesion and force transduction and is involved in signaling. The high-resolution molecular architecture of this layer is ...The glycocalyx, a highly heterogeneous glycoprotein layer of cilia regulates adhesion and force transduction and is involved in signaling. The high-resolution molecular architecture of this layer is currently not understood. The structure of the ciliary coat is described in the green alga Chlamydomonas reinhardtii by cryo-electron tomography and proteomic approaches and the high-resolution cryoEM structure of the main component, FMG1B is solved. FMG1B is described as a mucin orthologue which lacks the major O-glycosylation of mammalian mucins but is N-glycosylated. FMG1A, a previously undescribed isoform of FMG1B is expressed in C. reinhardtii. By microflow-based adhesion assays, increased surface adhesion in the glycocalyx deficient double-mutant fmg1b-fmg1a is observed. It is found this mutant is capable of surface-gliding, with neither isoform required for extracellular force transduction by intraflagellar transport. The results find FMG1 to form a protective layer with adhesion-regulative instead of adhesion-conferring properties and an example of an undescribed class of mucins.
External linksAdv Sci (Weinh) / PubMed:40041987 / PubMed Central
MethodsEM (single particle)
Resolution2.72 - 3.42 Å
Structure data

51499

9gos

EMDB-51499, PDB-9gos:
CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B.
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-51649: CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B. Local refinement 1 of 6.
Method: EM (single particle) / Resolution: 3.42 Å

EMDB-51650: CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B. Local refinement 2 of 6.
Method: EM (single particle) / Resolution: 3.28 Å

EMDB-51654: CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B. Local refinement 3 of 6.
Method: EM (single particle) / Resolution: 3.13 Å

EMDB-51655: CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B. Local refinement 4 of 6.
Method: EM (single particle) / Resolution: 2.72 Å

EMDB-51657: CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B. Local refinement 5 of 6.
Method: EM (single particle) / Resolution: 3.04 Å

EMDB-51658: CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B. Local refinement 6 of 6.
Method: EM (single particle) / Resolution: 3.04 Å

EMDB-51661: CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B. Low resolution consensus.
Method: EM (single particle) / Resolution: 3.24 Å

Source
  • chlamydomonas reinhardtii (plant)
KeywordsMEMBRANE PROTEIN / Mucin / Glycoprotein / Glycocalyx

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