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TitleCryo-EM reveals promoter DNA binding and conformational flexibility of the general transcription factor TFIID.
Journal, issue, pagesStructure, Vol. 17, Issue 11, Page 1442-1452, Year 2009
Publish dateNov 11, 2009
AuthorsHans Elmlund / Vera Baraznenok / Tomas Linder / Zsolt Szilagyi / Reza Rofougaran / Anders Hofer / Hans Hebert / Martin Lindahl / Claes M Gustafsson /
PubMed AbstractThe general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and ...The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the TFIID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.
External linksStructure / PubMed:19913479
MethodsEM (single particle)
Resolution8.0 - 10.0 Å
Structure data

EMDB-5134:
TBP-containing S. pombe TFIID
Method: EM (single particle) / Resolution: 8.0 Å

EMDB-5135:
TBP-lacking S. pombe TFIID
Method: EM (single particle) / Resolution: 10.0 Å

Source
  • S. pombe
  • Schizosaccharomyces pombe (fission yeast)

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