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TitleArchaeal type six secretion system mediates contact-dependent antagonism.
Journal, issue, pagesSci Adv, Vol. 10, Issue 46, Page eadp7088, Year 2024
Publish dateNov 15, 2024
AuthorsTobias Zachs / Jessie James L Malit / Jingwei Xu / Alexandra Schürch / Shamphavi Sivabalasarma / Phillip Nußbaum / Sonja-Verena Albers / Martin Pilhofer /
PubMed AbstractMicrobial communities are shaped by cell-cell interactions. Although archaea are often found in associations with other microorganisms, the mechanisms structuring these communities are poorly ...Microbial communities are shaped by cell-cell interactions. Although archaea are often found in associations with other microorganisms, the mechanisms structuring these communities are poorly understood. Here, we report on the structure and function of haloarchaeal contractile injection systems (CISs). Using a combination of functional assays and time-lapse imaging, we show that exhibits antagonism toward by inducing cell lysis and inhibiting proliferation. This antagonism is contact-dependent and requires a functional CIS, which is encoded by a gene cluster that is associated with toxin-immunity pairs. Cryo-focused ion beam milling and imaging by cryo-electron tomography revealed that these CISs are bound to the cytoplasmic membrane, resembling the bacterial type six secretion systems (T6SSs). We show that related T6SS gene clusters are conserved and expressed in other haloarchaeal strains, which exhibit antagonistic behavior. Our data provide a mechanistic framework for understanding how archaea may shape microbial communities and affect the food webs they inhabit.
External linksSci Adv / PubMed:39546591 / PubMed Central
MethodsEM (helical sym.) / EM (tomography) / EM (subtomogram averaging)
Resolution2.9 - 60.0 Å
Structure data

50089

9ezm

EMDB-50089, PDB-9ezm:
CryoEM structure of the contracted sheath in H. borinquense
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-51202: Cryo-electron tomogram of H. borinquense showing contracted T6SS complex
Method: EM (tomography)

EMDB-51203: Cryo-electron tomogram of H. borinquense showing extended T6SS complex
Method: EM (tomography)

EMDB-51204: Cryo-electron tomogram of H. elongans showing contracted T6SS complex
Method: EM (tomography)

EMDB-51205: Cryo-electron tomogram of H. elongans showing extended T6SS complex
Method: EM (tomography)

EMDB-51206: Sub-tomogram average of contracted sheath complex in H. borinquense with C6 symmetry.
Method: EM (subtomogram averaging) / Resolution: 60.0 Å

EMDB-51207: Sub-tomogram average of extended sheath complex in H. borinquense with C6 symmetry
Method: EM (subtomogram averaging) / Resolution: 60.0 Å

EMDB-51208: Sub-tomogram average of extended baseplate complex in H. borinquense without imposing symmetry
Method: EM (subtomogram averaging) / Resolution: 60.0 Å

EMDB-51209: Sub-tomogram average of extended baseplate complex in H. borinquense with C6 symmetry
Method: EM (subtomogram averaging) / Resolution: 60.0 Å

Source
  • halogeometricum borinquense dsm 11551 (archaea)
  • Haloferax elongans (archaea)
KeywordsSTRUCTURAL PROTEIN / archaea / contractile injection system / sheath / cryoEM

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