EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular mechanism of bacteriophage contraction structure of an S-layer-penetrating bacteriophage.
ジャーナル・号・ページ	Life Sci Alliance, Vol. 8, Issue 6, Year 2025
掲載日	2025年3月26日
著者	Jason S Wilson / Louis-Charles Fortier / Robert P Fagan / Per A Bullough /
PubMed 要旨	The molecular details of phage tail contraction and bacterial cell envelope penetration remain poorly understood and are completely unknown for phages infecting bacteria enveloped by proteinaceous S- ...The molecular details of phage tail contraction and bacterial cell envelope penetration remain poorly understood and are completely unknown for phages infecting bacteria enveloped by proteinaceous S-layers. Here, we reveal the extended and contracted atomic structures of an intact contractile-tailed phage (φCD508) that binds to and penetrates the protective S-layer of the Gram-positive human pathogen The tail is unusually long (225 nm), and it is also notable that the tail contracts less than those studied in related contractile injection systems such as the model phage T4 (∼20% compared with ∼50%). Surprisingly, we find no evidence of auxiliary enzymatic domains that other phages exploit in cell wall penetration, suggesting that sufficient energy is released upon tail contraction to penetrate the S-layer and the thick cell wall without enzymatic activity. Instead, the unusually long tail length, which becomes more flexible upon contraction, likely contributes toward the required free energy release for envelope penetration.
リンク	Life Sci Alliance / PubMed:40139691 / PubMed Central
手法	EM (単粒子) / EM (らせん対称)
解像度	2.64 - 4.11 Å
構造データ	51138 9g8s EMDB-51138, PDB-9g8s: C3 reconstruction of extended phiCD508 needle 手法: EM (単粒子) / 解像度: 3.96 Å 51191 9gay EMDB-51191, PDB-9gay: Extended phiCD508 capsid 手法: EM (単粒子) / 解像度: 3.74 Å 51192 9gaz EMDB-51192, PDB-9gaz: Contracted phiCD508 capsid 手法: EM (単粒子) / 解像度: 3.28 Å 51193 9gb0 EMDB-51193, PDB-9gb0: Extended phiCD508 portal adjacent capsid 手法: EM (単粒子) / 解像度: 3.23 Å 51194 9gb1 EMDB-51194, PDB-9gb1: Extended phiCD508 tail 手法: EM (らせん対称) / 解像度: 2.71 Å 51195 9gb2 EMDB-51195, PDB-9gb2: Extended phiCD508 baseplate 手法: EM (単粒子) / 解像度: 3.43 Å 51196 9gb3 EMDB-51196, PDB-9gb3: Extended phiCD508 portal 手法: EM (単粒子) / 解像度: 2.64 Å 51197 9gb4 EMDB-51197, PDB-9gb4: Contracted phiCD508 portal 手法: EM (単粒子) / 解像度: 2.88 Å 51198 9gb5 EMDB-51198, PDB-9gb5: Contracted phiCD508 neck 手法: EM (単粒子) / 解像度: 3.27 Å 51199 9gb6 EMDB-51199, PDB-9gb6: Contracted phiCD508 tail 手法: EM (らせん対称) / 解像度: 4.11 Å 51200 9gb7 EMDB-51200, PDB-9gb7: Extended phiCD508 neck 手法: EM (単粒子) / 解像度: 3.4 Å 51201 9gb8 EMDB-51201, PDB-9gb8: Contracted phiCD508 tail 手法: EM (単粒子) / 解像度: 4.11 Å
化合物	ChemComp-FE2: Unknown entry
由来	clostridioides difficile (バクテリア) clostridioides phage phicd508 (ファージ)
キーワード	VIRUS / Bacteriophage / needle / baseplate / capsid / VIRAL PROTEIN / portal / neck