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-Structure paper
| タイトル | Condensin II activation by M18BP1. |
|---|---|
| ジャーナル・号・ページ | Mol Cell, Vol. 85, Issue 14, Page 2688-2700.e11, Year 2025 |
| 掲載日 | 2025年7月17日 |
著者 | Alessandro Borsellini / Duccio Conti / Erin E Cutts / Rebecca J Harris / Kai Walstein / Andrea Graziadei / Valentina Cecatiello / Tom F Aarts / Ren Xie / Abdelghani Mazouzi / Sushweta Sen / Claire Hoencamp / Richard Pleuger / Sabrina Ghetti / Lina Oberste-Lehn / Dongqing Pan / Tanja Bange / Judith H I Haarhuis / Anastassis Perrakis / Thijn R Brummelkamp / Benjamin D Rowland / Andrea Musacchio / Alessandro Vannini / ![]() |
| PubMed 要旨 | Condensin I and II promote the drastic spatial rearrangement of the human genome upon mitotic entry. While condensin II is known to initiate this process in early mitosis, what triggers its ...Condensin I and II promote the drastic spatial rearrangement of the human genome upon mitotic entry. While condensin II is known to initiate this process in early mitosis, what triggers its activation and loading onto chromatin at this juncture remains unclear. Through genetic and proteomic approaches, we identify MIS18-binding protein 1 (M18BP1), a protein required to maintain centromere identity, as the elusive factor required for condensin II localization to chromatin. M18BP1 directly binds condensin II's CAP-G2 subunit. The condensin II antagonist MCPH1 also binds to CAP-G2 and outcompetes M18BP1 during interphase to maintain the genome in its uncondensed state. A switch from MCPH1 to M18BP1 at mitotic onset activates condensin II, thus promoting proper chromosome condensation. Regulation of this M18BP1-condensin interaction thus determines both the uncondensed state of the interphase genome and its compacted state in mitosis. |
リンク | Mol Cell / PubMed:40614722 |
| 手法 | EM (単粒子) |
| 解像度 | 7.5 Å |
| 構造データ | EMDB-50201, PDB-9f5w: |
| 由来 |
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キーワード | DNA BINDING PROTEIN / Chromosome organisation complex |
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homo sapiens (ヒト)
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