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TitleEconomical and Versatile Subunit Design Principles for Self-Assembled DNA Origami Structures.
Journal, issue, pagesACS Nano, Vol. 19, Issue 34, Page 30889-30901, Year 2025
Publish dateSep 2, 2025
AuthorsWei-Shao Wei / Thomas E Videbæk / Daichi Hayakawa / Rupam Saha / Juanita Pombo / Gaurav Arya / W Benjamin Rogers / Seth Fraden /
PubMed AbstractWe describe a modular design approach for creating versatile DNA origami subunits that can target diverse self-assembled structures. The subunit consists of a constant "core module" with variable ...We describe a modular design approach for creating versatile DNA origami subunits that can target diverse self-assembled structures. The subunit consists of a constant "core module" with variable "bond modules" and "angle modules" added to its exterior, controlling interaction specificity, strength, and structural geometry. The design features flexible joints between subunits, implemented by using single-stranded angle modules, whose mechanical properties and possible conformations are characterized by cryogenic electron microscopy and coarse-grained molecular modeling. We demonstrate the design's versatility through the assembly of structures with different Gaussian curvature, including sheets, spherical shells, and tubes. Our findings suggest that incorporating a judicious amount of flexibility in the bonds provides error tolerance in design and fabrication while maintaining target fidelity. Furthermore, off-target assemblies potentially introduced by flexibility can be counterbalanced by increasing the number of distinct bonds. This approach enables precise targeting of specific structural binding angles across a broad range of configurations by eliminating unfavorable interactions.
External linksACS Nano / PubMed:40830075 / PubMed Central
MethodsEM (single particle)
Resolution39.45 Å
Structure data

EMDB-49952: Dimerized subunit pair: modular DNA origami subunit with top = 14 poly-T, bottom = 3 poly-T overhangs
Method: EM (single particle) / Resolution: 39.45 Å

EMDB-49953: Dimerized subunit pair: modular DNA origami subunit with top = 3 poly-T, bottom = 3 poly-T overhangs
Method: EM (single particle) / Resolution: 39.45 Å

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