Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	An inhibitor of complement C5 provides structural insights into activation.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 117, Issue 1, Page 362-370, Year 2020
Publish date	Jan 7, 2020
Authors	Martin P Reichhardt / Steven Johnson / Terence Tang / Thomas Morgan / Nchimunya Tebeka / Niko Popitsch / Justin C Deme / Matthijs M Jore / Susan M Lea /
PubMed Abstract	The complement system is a crucial part of innate immune defenses against invading pathogens. The blood-meal of the tick lasts for days, and the tick must therefore rely on inhibitors to counter ...The complement system is a crucial part of innate immune defenses against invading pathogens. The blood-meal of the tick lasts for days, and the tick must therefore rely on inhibitors to counter complement activation. We have identified a class of inhibitors from tick saliva, the CirpT family, and generated detailed structural data revealing their mechanism of action. We show direct binding of a CirpT to complement C5 and have determined the structure of the C5-CirpT complex by cryoelectron microscopy. This reveals an interaction with the peripheral macro globulin domain 4 (C5_MG4) of C5. To achieve higher resolution detail, the structure of the C5_MG4-CirpT complex was solved by X-ray crystallography (at 2.7 Å). We thus present the fold of the CirpT protein family, and provide detailed mechanistic insights into its inhibitory function. Analysis of the binding interface reveals a mechanism of C5 inhibition, and provides information to expand our biological understanding of the activation of C5, and thus the terminal complement pathway.
External links	Proc Natl Acad Sci U S A / PubMed:31871188 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	2.7 - 3.5 Å
Structure data	4983 6rqj EMDB-4983, PDB-6rqj: Structure of human complement C5 complexed with tick inhibitors OmCI, RaCI1 and CirpT1 Method: EM (single particle) / Resolution: 3.5 Å PDB-6rpt: Structure of tick complement inhibitor CirpT1 complexed with macroglobubulin domain 4 of human complement C5 Method: X-RAY DIFFRACTION / Resolution: 2.7 Å
Chemicals	ChemComp-GOL: GLYCEROL ChemComp-HOH: WATER
Source	homo sapiens (human) Ornithodoros moubata (arthropod) Rhipicephalus appendiculatus (arthropod) rhipicephalus pulchellus (arthropod)
Keywords	IMMUNOSUPPRESSANT / complement / inhibitor / innate immunity / inflammation / C5 / terminal pathway inhibitor