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Structure paper

TitleStructural basis of RNA-guided transcription by a dCas12f-SigmaE-RNAP complex
Journal, issue, pagesNature, Year 2026
Publish dateFeb 10, 2025 (structure data deposition date)
AuthorsRenjian X / Hoffmann FT / Xie D / Wiegand T / Palmieri AI / Sternberg SH / Leifu C
External linksNature / Search PubMed
MethodsEM (single particle)
Resolution2.88 - 3.42 Å
Structure data

49165

9n9c

EMDB-49165, PDB-9n9c:
Cryo-EM structure of the dCas12f-gRNA complex
Method: EM (single particle) / Resolution: 3.28 Å

49173

9n9m

EMDB-49173, PDB-9n9m:
Cryo-EM structure of the dCas12f-gRNA-DNA complex (partial R-Loop)
Method: EM (single particle) / Resolution: 3.38 Å

49174

9n9o

EMDB-49174, PDB-9n9o:
Cryo-EM structure of the dCas12f-gRNA-dsDNA complex (full R-Loop)
Method: EM (single particle) / Resolution: 3.42 Å

49175

9n9p

EMDB-49175, PDB-9n9p:
Cryo-EM structure of the Fta RNAP complex
Method: EM (single particle) / Resolution: 2.95 Å

49176

9n9q

EMDB-49176, PDB-9n9q:
Cryo-EM structure of the RNA-guided transcription complex
Method: EM (single particle) / Resolution: 2.88 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • flagellimonas taeanensis (bacteria)
KeywordsRNA BINDING PROTEIN/RNA / dCas12f / binary complex / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex / RNA BINDING PROTEIN/DNA/RNA / partial R-Loop / RNA BINDING PROTEIN-DNA-RNA complex / Full R-Loop / Fta RNAP / RNA polymerase

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