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TitleInsights into type IX secretion from PorKN cogwheel structure bound to PorG and attachment complexes.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 7735, Year 2025
Publish dateAug 19, 2025
AuthorsDhana G Gorasia / Eric Hanssen / Manasi Mudaliyar / Craig J Morton / Sepideh Valimehr / Christine Seers / Lianyi Zhang / Matthew T Doyle / Debnath Ghosal / Paul D Veith / Eric C Reynolds /
PubMed AbstractThe Type IX Secretion System exports proteins across the outer membrane (OM) of bacteria in the Bacteroidota phylum, however, the mechanistic details remain unknown. In Porphyromonas gingivalis the ...The Type IX Secretion System exports proteins across the outer membrane (OM) of bacteria in the Bacteroidota phylum, however, the mechanistic details remain unknown. In Porphyromonas gingivalis the core components of the multi-protein complex are the Sov translocon, Attachment Complexes (PorQ, U, V, Z), PorLM molecular motors and PorKN rings. Here, we present a ~ 3.5 Å cryo-EM structure of the periplasmic rings comprising 32-33 subunits each of PorK and PorN. Additionally, we show the presence of a critical disulfide bond between PorK and the OM protein PorG that is essential for protein secretion and demonstrate that the Attachment Complexes bind to, and are localized above, the PorKN rings. Overall, each ring resembles a cogwheel with PorN forming cog-like projections that we propose engage with the PorLM motor to drive the rotation of the PorKN cogwheel together with PorG and associated Attachment Complexes, thus providing the energy to complete protein secretion and the coordinated cell surface attachment of the secreted cargo.
External linksNat Commun / PubMed:40830366 / PubMed Central
MethodsEM (single particle)
Resolution2.75 - 3.6 Å
Structure data

EMDB-48722: cryo electron microscopy map of porK/N ring with alternative C32 symmetry
Method: EM (single particle) / Resolution: 3.6 Å

48741

9myj

EMDB-48741, PDB-9myj:
Structure of P. gingivalis PorK and PorN complexes from cryo electron microscopy
Method: EM (single particle) / Resolution: 3.57 Å

71309

9p6h

EMDB-71309, PDB-9p6h:
Structure of P. gingivalis PorK and PorN complexes from cryo electron microscopy
Method: EM (single particle) / Resolution: 2.75 Å

Chemicals

ChemComp-MAN:
alpha-D-mannopyranose

ChemComp-CA:
Unknown entry

ChemComp-BMA:
beta-D-mannopyranose

Source
  • Porphyromonas gingivalis (bacteria)
  • porphyromonas gingivalis w83 (bacteria)
KeywordsTRANSPORT PROTEIN / T9SS / porphyromonas gingivalis

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