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-Structure paper
| Title | Structure of yeast RAVE bound to a partial V complex. |
|---|---|
| Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 121, Issue 50, Page e2414511121, Year 2024 |
| Publish date | Dec 10, 2024 |
 Authors | Hanlin Wang / Maureen Tarsio / Patricia M Kane / John L Rubinstein /   |
| PubMed Abstract | Vacuolar-type ATPases (V-ATPases) are membrane-embedded proton pumps that acidify intracellular compartments in almost all eukaryotic cells. Homologous with ATP synthases, these multisubunit enzymes ...Vacuolar-type ATPases (V-ATPases) are membrane-embedded proton pumps that acidify intracellular compartments in almost all eukaryotic cells. Homologous with ATP synthases, these multisubunit enzymes consist of a soluble catalytic V subcomplex and a membrane-embedded proton-translocating V subcomplex. The V and V subcomplexes can undergo reversible dissociation to regulate proton pumping, with reassociation of V and V requiring the protein complex known as RAVE (regulator of the ATPase of vacuoles and endosomes). In the yeast , RAVE consists of subunits Rav1p, Rav2p, and Skp1p. We used electron cryomicroscopy (cryo-EM) to determine a structure of yeast RAVE bound to V. In the structure, RAVE is an L-shaped complex with Rav2p pointing toward the membrane and Skp1p distant from both the membrane and V. Only Rav1p interacts with V, binding to a region of subunit A not found in the corresponding ATP synthase subunit. When bound to RAVE, V is in a rotational state suitable for binding the free V complex, but in the structure, it is partially disrupted, missing five of its 16 subunits. Other than these missing subunits and the conformation of the inhibitory subunit H, the V complex with RAVE appears poised for reassembly with V. |
 External links | Proc Natl Acad Sci U S A / PubMed:39625975 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 2.7 - 12.0 Å |
| Structure data | EMDB-45788, PDB-9cop:  EMDB-45789: Yeast RAVE complex  EMDB-46462: Locally refined map of yeast RAVE complex  EMDB-46463: Consensus map of yeast RAVE bound to V-ATPase V1 |
| Chemicals |  ChemComp-MG:  ChemComp-ADP: |
| Source |
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 Keywords | HYDROLASE / V-ATPase / RAVE / assembly |
saccharomyces cerevisiae (brewer's yeast)