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TitleHigh-throughput synthesis and specificity characterization of natively paired influenza hemagglutinin antibodies with oPool display.
Journal, issue, pagesSci Transl Med, Vol. 17, Issue 814, Page eadt4147, Year 2025
Publish dateSep 3, 2025
AuthorsWenhao O Ouyang / Huibin Lv / Wenkan Liu / Ruipeng Lei / Zongjun Mou / Tossapol Pholcharee / Logan Talmage / Meixuan Tong / Wei Ji / Yiquan Wang / Katrine E Dailey / Akshita B Gopal / Danbi Choi / Madison R Ardagh / Lucia A Rodriguez / Jenna J Guthmiller / Xinghong Dai / Nicholas C Wu /
PubMed AbstractAntibody discovery is crucial for developing therapeutics and vaccines and for understanding adaptive immunity. However, the lack of approaches to synthesize antibodies with defined sequences in a ...Antibody discovery is crucial for developing therapeutics and vaccines and for understanding adaptive immunity. However, the lack of approaches to synthesize antibodies with defined sequences in a high-throughput manner represents a major bottleneck in antibody discovery. Here, we present oPool display, a high-throughput cell-free platform that combined oligo pool synthesis and mRNA display to rapidly construct and characterize hundreds to thousands of natively paired antibodies in parallel. As a proof of concept, we applied oPool display to probe the binding specificity of more than 300 uncommon influenza hemagglutinin-specific antibodies against nine hemagglutinin variants through 16 screens. More than 5000 binding tests were performed in 3 to 5 days of hands-on time with further scaling potential. Follow-up structural and functional analysis of two antibodies revealed the versatility of the human immunoglobulin gene segment D3-3 () in recognizing the hemagglutinin stem. Overall, this study established an experimental platform that not only accelerates antibody characterization but also enables unbiased discovery of recurring molecular signatures among antibodies with the same specificity.
External linksSci Transl Med / PubMed:40901925 / PubMed Central
MethodsEM (single particle)
Resolution2.82 - 2.9 Å
Structure data

45930

9cu7

EMDB-45930, PDB-9cu7:
Structure of 16.ND.92 Fab in complex with A/Solomon Islands/3/2006(H1N1) influenza virus Hemagglutinin
Method: EM (single particle) / Resolution: 2.82 Å

46727

9dbx

EMDB-46727, PDB-9dbx:
Structure of AG11-2F01 Fab in complex with A/Solomon Islands/3/2006 (H1N1) influenza virus hemagglutinin
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
  • influenza a virus (a/solomon islands/3/2006(h1n1))
  • influenza a virus
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / Antibody / Influenza / Hemagglutinin / VIRAL PROTEIN-IMMUNE SYSTEM complex / antibody-antigen complex

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