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TitleComplete structures of the YenTc holotoxin prepore and pore reveal the evolutionary basis for chitinase incorporation into ABC toxins.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 11121, Year 2025
Publish dateDec 15, 2025
AuthorsYu Shang Low / Solace G Roche / Nadezhda A Aleksandrova / Gabriel Foley / Jason Kk Low / Joseph K Box / Tristan I Croll / Irene R Chassagnon / J Shaun Lott / Evelyne Deplazes / Mikael Bodén / Mark Rh Hurst / Sarah J Piper / Michael J Landsberg /
PubMed AbstractABC toxins are toxin-translocating, pore-forming proteins found in a wide range of insecticidal bacteria and some mammalian pathogens. The Yersinia entomopahaga toxin complex (YenTc) belongs to a ...ABC toxins are toxin-translocating, pore-forming proteins found in a wide range of insecticidal bacteria and some mammalian pathogens. The Yersinia entomopahaga toxin complex (YenTc) belongs to a distinct subclass of ABC toxins, defined by a divergent molecular architecture. Structural details that define their mechanism of action remain to be elucidated. Here we determine structures of the YenTc holotoxin assembly in both prepore and pore-forming configurations using cryo-EM in conjunction with Alphafold2-assisted structural modelling of flexible domains. We define the structural mechanism via which enzymatically-active chitinase subunits are incorporated, and show using phylogenetic analyses that this subclass-defining feature has evolved relatively recently. Our structures point to the existence of distinct conformational states in YenTc, which may distinguish it from other structurally-characterised ABC toxins, or represent states on a shared mechanistic trajectory. Thus, our findings enhance our understanding of the structural diversity that defines distinct ABC toxin subclasses.
External linksNat Commun / PubMed:41397958 / PubMed Central
MethodsEM (single particle)
Resolution4.6 - 6.43 Å
Structure data

45190

9c4k

EMDB-45190, PDB-9c4k:
Yersinia entomophaga holotoxin complex in prepore conformation
Method: EM (single particle) / Resolution: 4.6 Å

45422

9cbc

EMDB-45422, PDB-9cbc:
Yersinia entomophaga holotoxin complex in pore conformation
Method: EM (single particle) / Resolution: 6.43 Å

EMDB-45423: Yersinia entomophaga toxin complex TcA subunit
Method: EM (single particle) / Resolution: 5.9 Å

Source
  • yersinia entomophaga (bacteria)
KeywordsTOXIN / pore-forming / insecticidal / chitinases

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