EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	STK19 positions TFIIH for cell-free transcription-coupled DNA repair.
ジャーナル・号・ページ	Cell, Vol. 187, Issue 25, Page 7091-7106.e24, Year 2024
掲載日	2024年12月12日
著者	Tycho E T Mevissen / Maximilian Kümmecke / Ernst W Schmid / Lucas Farnung / Johannes C Walter /
PubMed 要旨	In transcription-coupled nucleotide excision repair (TC-NER), stalled RNA polymerase II (RNA Pol II) binds CSB and CRL4, which cooperate with UVSSA and ELOF1 to recruit TFIIH. To explore the ...In transcription-coupled nucleotide excision repair (TC-NER), stalled RNA polymerase II (RNA Pol II) binds CSB and CRL4, which cooperate with UVSSA and ELOF1 to recruit TFIIH. To explore the mechanism of TC-NER, we recapitulated this reaction in vitro. When a plasmid containing a site-specific lesion is transcribed in frog egg extract, error-free repair is observed that depends on CSB, CRL4, UVSSA, and ELOF1. Repair also requires STK19, a factor previously implicated in transcription recovery after UV exposure. A 1.9-Å cryo-electron microscopy structure shows that STK19 binds the TC-NER complex through CSA and the RPB1 subunit of RNA Pol II. Furthermore, AlphaFold predicts that STK19 interacts with the XPD subunit of TFIIH, and disrupting this interface impairs cell-free repair. Molecular modeling suggests that STK19 positions TFIIH ahead of RNA Pol II for lesion verification. Our analysis of cell-free TC-NER suggests that STK19 couples RNA Pol II stalling to downstream repair events.
リンク	Cell / PubMed:39547228 / PubMed Central
手法	EM (単粒子)
解像度	1.9 - 2.7 Å
構造データ	45050 9bz0 EMDB-45050, PDB-9bz0: Structure of an STK19-containing TC-NER complex 手法: EM (単粒子) / 解像度: 1.9 Å EMDB-47261: Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map IV) 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-47262: Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map III) 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-47263: Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map II) 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-47266: Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map V) 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-47267: Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map VI) 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-47271: Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map VII) 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-47272: Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (map VIII) 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-47273: Cryo-EM reconstruction of TC-NER transcription elongation complex with STK19 (Consensus refinement) 手法: EM (単粒子) / 解像度: 1.9 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER
由来	homo sapiens (ヒト) sus scrofa (ブタ) synthetic construct (人工物)
キーワード	TRANSCRIPTION/DNA/RNA / DNA repair / RNA polymerase II / Co-transcriptional process / TRANSCRIPTION / TRANSCRIPTION-DNA-RNA complex