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TitleDynamic fibrillar assembly of αB-crystallin induced by perturbation of the conserved NT-IXI motif resolved by cryo-EM.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 10336, Year 2024
Publish dateNov 28, 2024
AuthorsRussell McFarland / Rozhan Noroozi / Adam P Miller / Steve L Reichow /
PubMed AbstractαB-crystallin is an archetypical member of the small heat shock proteins (sHSPs) vital for cellular proteostasis and mitigating protein misfolding diseases. Gaining insights into the principles ...αB-crystallin is an archetypical member of the small heat shock proteins (sHSPs) vital for cellular proteostasis and mitigating protein misfolding diseases. Gaining insights into the principles defining their molecular organization and chaperone function have been hindered by intrinsic dynamic properties and limited high-resolution structural analysis. To disentangle the mechanistic underpinnings of these dynamical properties, we ablate a conserved IXI-motif located within the N-terminal (NT) domain of human αB-crystallin implicated in subunit exchange dynamics and client sequestration. This results in a profound structural transformation, from highly polydispersed caged-like native assemblies into an elongated fibril state amenable to high-resolution cryo-EM analysis. The reversible nature of this variant facilitates interrogation of functional effects due to perturbation of the NT-IXI motif in both the native-like oligomer and fibril states. Together, our investigations unveil several features thought to be key mechanistic attributes to sHSPs and point to a critical significance of the NT-IXI motif in αB-crystallin assembly, polydispersity, and chaperone activity.
External linksNat Commun / PubMed:39609421 / PubMed Central
MethodsEM (helical sym.)
Resolution3.4 Å
Structure data

44477

9bee

EMDB-44477, PDB-9bee:
alphaB-crystallin N-terminal IXI variant in a fibril state
Method: EM (helical sym.) / Resolution: 3.4 Å

Source
  • homo sapiens (human)
KeywordsCHAPERONE / small heat-shock protein fibril proteostasis cataract

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