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TitleCryo-electron tomographic investigation of native hippocampal glutamatergic synapses.
Journal, issue, pagesElife, Vol. 13, Year 2024
Publish dateNov 4, 2024
AuthorsAya Matsui / Cathy Spangler / Johannes Elferich / Momoko Shiozaki / Nikki Jean / Xiaowei Zhao / Maozhen Qin / Haining Zhong / Zhiheng Yu / Eric Gouaux /
PubMed AbstractChemical synapses are the major sites of communication between neurons in the nervous system and mediate either excitatory or inhibitory signaling. At excitatory synapses, glutamate is the primary ...Chemical synapses are the major sites of communication between neurons in the nervous system and mediate either excitatory or inhibitory signaling. At excitatory synapses, glutamate is the primary neurotransmitter and upon release from presynaptic vesicles, is detected by postsynaptic glutamate receptors, which include ionotropic AMPA and NMDA receptors. Here, we have developed methods to identify glutamatergic synapses in brain tissue slices, label AMPA receptors with small gold nanoparticles (AuNPs), and prepare lamella for cryo-electron tomography studies. The targeted imaging of glutamatergic synapses in the lamella is facilitated by fluorescent pre- and postsynaptic signatures, and the subsequent tomograms allow for the identification of key features of chemical synapses, including synaptic vesicles, the synaptic cleft, and AuNP-labeled AMPA receptors. These methods pave the way for imaging brain regions at high resolution, using unstained, unfixed samples preserved under near-native conditions.
External linksElife / PubMed:39495821 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-44174: Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses - Tomogram 1
Method: EM (tomography)

EMDB-44175: Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses - Tomogram 2
Method: EM (tomography)

EMDB-44176: Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses - Tomogram 3
Method: EM (tomography)

Source
  • Mus musculus (house mouse)

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