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TitleRIP2 filament formation is required for NOD2 dependent NF-κB signalling.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 4043, Year 2018
Publish dateOct 2, 2018
AuthorsErika Pellegrini / Ambroise Desfosses / Arndt Wallmann / Wiebke Manuela Schulze / Kristina Rehbein / Philippe Mas / Luca Signor / Stephanie Gaudon / Grasilda Zenkeviciute / Michael Hons / Helene Malet / Irina Gutsche / Carsten Sachse / Guy Schoehn / Hartmut Oschkinat / Stephen Cusack /
PubMed AbstractActivation of the innate immune pattern recognition receptor NOD2 by the bacterial muramyl-dipeptide peptidoglycan fragment triggers recruitment of the downstream adaptor kinase RIP2, eventually ...Activation of the innate immune pattern recognition receptor NOD2 by the bacterial muramyl-dipeptide peptidoglycan fragment triggers recruitment of the downstream adaptor kinase RIP2, eventually leading to NF-κB activation and proinflammatory cytokine production. Here we show that full-length RIP2 can form long filaments mediated by its caspase recruitment domain (CARD), in common with other innate immune adaptor proteins. We further show that the NOD2 tandem CARDs bind to one end of the RIP2 CARD filament, suggesting a mechanism for polar filament nucleation by activated NOD2. We combine X-ray crystallography, solid-state NMR and high-resolution cryo-electron microscopy to determine the atomic structure of the helical RIP2 CARD filament, which reveals the intermolecular interactions that stabilize the assembly. Using structure-guided mutagenesis, we demonstrate the importance of RIP2 polymerization for the activation of NF-κB signalling by NOD2. Our results could be of use to develop new pharmacological strategies to treat inflammatory diseases characterised by aberrant NOD2 signalling.
External linksNat Commun / PubMed:30279485 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution3.3 - 3.94 Å
Structure data

4399

6ggs

EMDB-4399: Cryo-EM structure of the RIP2 CARD filament
PDB-6ggs: Structure of RIP2 CARD filament
Method: EM (helical sym.) / Resolution: 3.94 Å

PDB-6gfj:
Structure of RIP2 CARD domain fused to crystallisable MBP tag
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

Source
  • homo sapiens (human)
  • methanosarcina mazei (archaea)
KeywordsTRANSFERASE / CARD / crystallographic MBP / RIP2 / Death Domain / filament / helical

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