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-Structure paper
| タイトル | Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain. |
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| ジャーナル・号・ページ | Sci Adv, Vol. 10, Issue 48, Page eadp4937, Year 2024 |
| 掲載日 | 2024年11月29日 |
著者 | Christopher J Gisriel / Vasily Kurashov / David F Iwig / Brandon P Russell / David J Vinyard / Gary W Brudvig / John H Golbeck / K V Lakshmi / ![]() |
| PubMed 要旨 | Photosystem I (PS I) is a light-driven oxidoreductase responsible for converting photons into chemical bond energy. Its application for renewable energy was revolutionized by the creation of the MenB ...Photosystem I (PS I) is a light-driven oxidoreductase responsible for converting photons into chemical bond energy. Its application for renewable energy was revolutionized by the creation of the MenB deletion (Δ) variant in the cyanobacterium sp. PCC 6803, in which phylloquinone is replaced by plastoquinone-9 with a low binding affinity. This permits its exchange with exogenous quinones covalently coupled to dihydrogen catalysts that bind with high affinity, thereby converting PS I into a stable solar fuel catalyst. Here, we reveal the 2.03-Å-resolution cryo-EM structure of a recent MenB variant of PS I. The quinones and their binding environment are analyzed in the context of previous biophysical data, thereby enabling a protocol to solve future PS I hybrids and constructs from this genetically tractable cyanobacterium. |
リンク | Sci Adv / PubMed:39612342 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.03 Å |
| 構造データ | EMDB-43843, PDB-9au4: |
| 化合物 | ![]() ChemComp-CLA: ![]() PDB-1agd: ![]() ChemComp-SF4: ![]() ChemComp-BCR: ![]() ChemComp-LHG: ![]() ChemComp-LMG: ![]() ChemComp-45D: ![]() ChemComp-CL: ![]() ChemComp-ECH: ![]() ChemComp-CA: ![]() ChemComp-EQ3: ![]() ChemComp-ZEX: ![]() ChemComp-SQD: ![]() ChemComp-DGD: ![]() ChemComp-LMT: ![]() ChemComp-HOH: |
| 由来 |
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キーワード | PHOTOSYNTHESIS / cyanobacteria / photosystem I / MenB / phylloquinone / plastoquinone / benzoquinone / naphthoquinone / redox potential |
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