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TitleGABA receptor π forms channels that stimulate ERK through a G-protein-dependent pathway.
Journal, issue, pagesMol Cell, Vol. 85, Issue 1, Page 166-176.e5, Year 2025
Publish dateJan 2, 2025
AuthorsYueyue Wang / Yalan Zhang / Wenxue Li / Barbora Salovska / Jianan Zhang / Tongqing Li / Hengyi Li / Yansheng Liu / Leonard K Kaczmarek / Lajos Pusztai / Daryl E Klein /
PubMed AbstractThe rare γ-aminobutyric acid type-A receptor (GABAR) subunit π (GABRP) is highly expressed in certain cancers, where it stimulates growth through extracellular-regulated kinase (ERK) signaling by ...The rare γ-aminobutyric acid type-A receptor (GABAR) subunit π (GABRP) is highly expressed in certain cancers, where it stimulates growth through extracellular-regulated kinase (ERK) signaling by an uncharacterized pathway. To elucidate GABRP's signaling mechanism, we determined cryoelectron microscopy (cryo-EM) structures of GABRP embedded in native nanodiscs, both in the presence and absence of GABA. Structurally, GABRP homopentamers closely resemble heteropentameric GABAR anion channels, transitioning from a closed "resting" state to an open "active" state upon GABA binding. However, functional assays reveal that GABRP responds more like a type-B metabotropic receptor. At physiological concentrations of GABA, chloride flux is not detected. Rather, GABRP activates a G-protein-coupled pathway leading to ERK signaling. Ionotropic activity is only triggered at supraphysiological GABA concentrations, effectively decoupling it from GABRP's signaling functions. These findings provide a structural and functional blueprint for GABRP, opening new avenues for targeted inhibition of GABA growth signals in GABRP-positive cancers.
External linksMol Cell / PubMed:39642883 / PubMed Central
MethodsEM (single particle)
Resolution3.07 - 3.1 Å
Structure data

43512

8vsz

EMDB-43512, PDB-8vsz:
CryoEM structure of human GABAA receptor pi (GABRP) apo state
Method: EM (single particle) / Resolution: 3.07 Å

43546

8vv0

EMDB-43546, PDB-8vv0:
CryoEM structure of human GABAA receptor pi (GABRP) in complex with GABA
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-ABU:
GAMMA-AMINO-BUTANOIC ACID / neurotransmitter, inhibitor*YM

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / Cryo-EM / GABAA receptor / Channel / complex

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