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TitleStructure of the Portal Complex from Staphylococcus aureus Pathogenicity Island 1 Transducing Particles In Situ and In Isolation.
Journal, issue, pagesJ Mol Biol, Vol. 436, Issue 4, Page 168415, Year 2024
Publish dateDec 21, 2023
AuthorsAmarshi Mukherjee / James L Kizziah / N'Toia C Hawkins / Mohamed O Nasef / Laura K Parker / Terje Dokland /
PubMed AbstractStaphylococcus aureus is an important human pathogen, and the prevalence of antibiotic resistance is a major public health concern. The evolution of pathogenicity and resistance in S. aureus often ...Staphylococcus aureus is an important human pathogen, and the prevalence of antibiotic resistance is a major public health concern. The evolution of pathogenicity and resistance in S. aureus often involves acquisition of mobile genetic elements (MGEs). Bacteriophages play an especially important role, since transduction represents the main mechanism for horizontal gene transfer. S. aureus pathogenicity islands (SaPIs), including SaPI1, are MGEs that carry genes encoding virulence factors, and are mobilized at high frequency through interactions with specific "helper" bacteriophages, such as 80α, leading to packaging of the SaPI genomes into virions made from structural proteins supplied by the helper. Among these structural proteins is the portal protein, which forms a ring-like portal at a fivefold vertex of the capsid, through which the DNA is packaged during virion assembly and ejected upon infection of the host. We have used high-resolution cryo-electron microscopy to determine structures of the S. aureus bacteriophage 80α portal itself, produced by overexpression, and in situ in the empty and full SaPI1 virions, and show how the portal interacts with the capsid. These structures provide a basis for understanding portal and capsid assembly and the conformational changes that occur upon DNA packaging and ejection.
External linksJ Mol Biol / PubMed:38135177 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 3.5 Å
Structure data

43033

8v8b

EMDB-43033, PDB-8v8b:
Structure of 80alpha portal protein expressed in E. coli
Method: EM (single particle) / Resolution: 2.4 Å

43142

8vd4

EMDB-43142, PDB-8vd4:
SaPI1 mature capsid structure containing DNA
Method: EM (single particle) / Resolution: 3.1 Å

43143

8vd5

EMDB-43143, PDB-8vd5:
SaPI1 mature capsid structure without DNA
Method: EM (single particle) / Resolution: 3.2 Å

43145

8vd8

EMDB-43145, PDB-8vd8:
SaPI1 portal structure in mature capsids containing DNA
Method: EM (single particle) / Resolution: 3.2 Å

43146

8vdc

EMDB-43146, PDB-8vdc:
SaPI1 portal structure in mature capsids without DNA
Method: EM (single particle) / Resolution: 3.5 Å

43147

8vde

EMDB-43147, PDB-8vde:
SaPI1 portal-capsid interface in mature capsids with DNA
Method: EM (single particle) / Resolution: 3.4 Å

Source
  • staphylococcus phage 80alpha (virus)
  • dubowvirus dv80alpha
KeywordsSTRUCTURAL PROTEIN / complex / phage / sapi / portal / VIRUS LIKE PARTICLE / capsid

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