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| Title | Correlative Microscopy of Vitreous Sections Provides Insights into BAR-Domain Organization In Situ. |
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| Journal, issue, pages | Structure, Vol. 26, Issue 6, Page 879-886.e3, Year 2018 |
| Publish date | Jun 5, 2018 |
 Authors | Tanmay A M Bharat / Patrick C Hoffmann / Wanda Kukulski /  |
| PubMed Abstract | Electron microscopy imaging of macromolecular complexes in their native cellular context is limited by the inherent difficulty to acquire high-resolution tomographic data from thick cells and to ...Electron microscopy imaging of macromolecular complexes in their native cellular context is limited by the inherent difficulty to acquire high-resolution tomographic data from thick cells and to specifically identify elusive structures within crowded cellular environments. Here, we combined cryo-fluorescence microscopy with electron cryo-tomography of vitreous sections into a coherent correlative microscopy workflow, ideal for detection and structural analysis of elusive protein assemblies in situ. We used this workflow to address an open question on BAR-domain coating of yeast plasma membrane compartments known as eisosomes. BAR domains can sense or induce membrane curvature, and form scaffold-like membrane coats in vitro. Our results demonstrate that in cells, the BAR protein Pil1 localizes to eisosomes of varying membrane curvature. Sub-tomogram analysis revealed a dense protein coat on curved eisosomes, which was not present on shallow eisosomes, indicating that while BAR domains can assemble at shallow membranes in vivo, scaffold formation is tightly coupled to curvature generation. |
 External links | Structure / PubMed:29681471 / PubMed Central |
| Methods | EM (tomography) |
| Structure data |  EMDB-4305: |
| Source |
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Saccharomyces cerevisiae S288c (yeast)