EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	RNA control of reverse transcription in a diversity-generating retroelement.
ジャーナル・号・ページ	Nature, Vol. 638, Issue 8052, Page 1122-1129, Year 2025
掲載日	2025年1月8日
著者	Sumit Handa / Tapan Biswas / Jeet Chakraborty / Gourisankar Ghosh / Blair G Paul / Partho Ghosh /
PubMed 要旨	Diversity-generating retroelements (DGRs) create massive protein sequence variation (up to 10) in ecologically diverse microorganisms. A recent survey identified around 31,000 DGRs from more than ...Diversity-generating retroelements (DGRs) create massive protein sequence variation (up to 10) in ecologically diverse microorganisms. A recent survey identified around 31,000 DGRs from more than 1,500 bacterial and archaeal genera, constituting more than 90 environment types. DGRs are especially enriched in the human gut microbiome and nano-sized microorganisms that seem to comprise most microbial life and maintain DGRs despite reduced genomes. DGRs are also implicated in the emergence of multicellularity. Variation occurs during reverse transcription of a protein-encoding RNA template coupled to misincorporation at adenosines. In the prototypical Bordetella bacteriophage DGR, the template must be surrounded by upstream and downstream RNA segments for complementary DNA synthesis to be carried out by a complex of the DGR reverse transcriptase bRT and associated protein Avd. The function of the surrounding RNA was unknown. Here we show through cryogenic electron microscopy that this RNA envelops bRT and lies over the barrel-shaped Avd, forming an intimate ribonucleoprotein. An abundance of essential interactions in the ribonucleoprotein precisely position an RNA homoduplex in the bRT active site for initiation of reverse transcription. Our results explain how the surrounding RNA primes complementary DNA synthesis, promotes processivity, terminates polymerization and strictly limits mutagenesis to specific proteins through mechanisms that are probably conserved in DGRs belonging to distant taxa.
リンク	Nature / PubMed:39779855 / PubMed Central
手法	EM (単粒子)
解像度	3.05 - 3.61 Å
構造データ	42077 8ub7 EMDB-42077, PDB-8ub7: Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Active state (N-occupied) 手法: EM (単粒子) / 解像度: 3.2 Å 42078 8ub8 EMDB-42078, PDB-8ub8: Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Pre-active State 1a 手法: EM (単粒子) / 解像度: 3.28 Å 42079 8ub9 EMDB-42079, PDB-8ub9: Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase- Active state (N-empty) 1a 手法: EM (単粒子) / 解像度: 3.07 Å 42080 8uba EMDB-42080, PDB-8uba: Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Pre-active state 1b 手法: EM (単粒子) / 解像度: 3.2 Å 42081 8ubb EMDB-42081, PDB-8ubb: Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Active State (N-empty) 1b 手法: EM (単粒子) / 解像度: 3.23 Å 42082 8ubc EMDB-42082, PDB-8ubc: Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Resting State 1b 手法: EM (単粒子) / 解像度: 3.29 Å 42083 8ubd EMDB-42083, PDB-8ubd: Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Pre-active State 2 手法: EM (単粒子) / 解像度: 3.05 Å 42084 8ube EMDB-42084, PDB-8ube: Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Resting State 1a 手法: EM (単粒子) / 解像度: 3.05 Å 42085 8ubf EMDB-42085, PDB-8ubf: Diversity-generating retroelement (DGR) ribonucleoprotein - Resting state 1c 手法: EM (単粒子) / 解像度: 3.61 Å
化合物	ChemComp-DCP: 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE ChemComp-MG: Unknown entry
由来	bordetella phage bpp-1 (ファージ) methylorubrum extorquens (バクテリア)
キーワード	RNA BINDING PROTEIN/RNA / Reverse transcriptase / Ribonucleoprotein / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex