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TitleStructure of the core of the type III secretion system export apparatus.
Journal, issue, pagesNat Struct Mol Biol, Vol. 25, Issue 7, Page 583-590, Year 2018
Publish dateJul 2, 2018
AuthorsLucas Kuhlen / Patrizia Abrusci / Steven Johnson / Joseph Gault / Justin Deme / Joseph Caesar / Tobias Dietsche / Mehari Tesfazgi Mebrhatu / Tariq Ganief / Boris Macek / Samuel Wagner / Carol V Robinson / Susan M Lea /
PubMed AbstractExport of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are ...Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are suggested to form the core of an export gate in the inner membrane, but their structure, assembly and location within the final nanomachine remain unclear. Here, we present the cryoelectron microscopy structure of the Salmonella Typhimurium FliP-FliQ-FliR complex at 4.2 Å. None of the subunits adopt canonical integral membrane protein topologies, and common helix-turn-helix structural elements allow them to form a helical assembly with 5:4:1 stoichiometry. Fitting of the structure into reconstructions of intact secretion systems, combined with cross-linking, localize the export gate as a core component of the periplasmic portion of the machinery. This study thereby identifies the export gate as a key element of the secretion channel and implies that it primes the helical architecture of the components assembling downstream.
External linksNat Struct Mol Biol / PubMed:29967543 / PubMed Central
MethodsEM (single particle)
Resolution4.2 Å
Structure data

4173

6f2d

EMDB-4173, PDB-6f2d:
A FliPQR complex forms the core of the Salmonella type III secretion system export apparatus.
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • Salmonella enterica (bacteria)
  • salmonella enterica subsp. enterica (bacteria)
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
KeywordsPROTEIN TRANSPORT / T3SS / Flagella / Cryo-EM / Export / Membrane protein

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