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TitleThe RAD52 double-ring remodels replication forks restricting fork reversal.
Journal, issue, pagesNature, Vol. 641, Issue 8062, Page 512-519, Year 2025
Publish dateApr 2, 2025
AuthorsMasayoshi Honda / Mortezaali Razzaghi / Paras Gaur / Eva Malacaria / Giorgia Marozzi / Ludovica Di Biagi / Francesca Antonella Aiello / Emeleeta A Paintsil / Andrew J Stanfield / Bailey J Deppe / Lokesh Gakhar / Nicholas J Schnicker / M Ashley Spies / Pietro Pichierri / Maria Spies /
PubMed AbstractHuman RAD52 is a multifunctional DNA repair protein involved in several cellular events that support genome stability, including protection of stalled DNA replication forks from excessive degradation. ...Human RAD52 is a multifunctional DNA repair protein involved in several cellular events that support genome stability, including protection of stalled DNA replication forks from excessive degradation. In its gatekeeper role, RAD52 binds to and stabilizes stalled replication forks during replication stress, protecting them from reversal by SMARCAL1 motor. The structural and molecular mechanism of the RAD52-mediated fork protection remains elusive. Here, using P1 nuclease sensitivity, biochemical and single-molecule analyses, we show that RAD52 dynamically remodels replication forks through its strand exchange activity. The presence of the single-stranded DNA binding protein RPA at the fork modulates the kinetics of the strand exchange without impeding the reaction outcome. Mass photometry and single-particle cryo-electron microscopy show that the replication fork promotes a unique nucleoprotein structure containing head-to-head arrangement of two undecameric RAD52 rings with an extended positively charged surface that accommodates all three arms of the replication fork. We propose that the formation and continuity of this surface is important for the strand exchange reaction and for competition with SMARCAL1.
External linksNature / PubMed:40175552 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 8.5 Å
Structure data

41357

8tkq

EMDB-41357, PDB-8tkq:
Cryo-EM structure of human full-length RAD52
Method: EM (single particle) / Resolution: 2.5 Å

EMDB-42065: Cryo-EM structure of human full-length RAD52 in the presence of fork DNA
Method: EM (single particle) / Resolution: 6.1 Å

EMDB-42066: Cryo-EM density map of a double-ring of human RAD52 in the presence of fork DNA
Method: EM (single particle) / Resolution: 5.32 Å

EMDB-42069: Cryo-EM density map of a double-ring of human RAD52 in the presence of fork DNA
Method: EM (single particle) / Resolution: 8.5 Å

EMDB-42440: Cryo-EM structure of human full-length RAD52 in the presence of fork DNA
Method: EM (single particle) / Resolution: 6.75 Å

Source
  • homo sapiens (human)
KeywordsRECOMBINATION / DNA repair

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