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TitleCryo-EM structure of respiratory complex I reveals a link to mitochondrial sulfur metabolism.
Journal, issue, pagesBiochim Biophys Acta, Vol. 1857, Issue 12, Page 1935-1942, Year 2016
Publish dateSep 30, 2016
AuthorsEdoardo D'Imprima / Deryck J Mills / Kristian Parey / Ulrich Brandt / Werner Kühlbrandt / Volker Zickermann / Janet Vonck /
PubMed AbstractMitochondrial complex I is a 1MDa membrane protein complex with a central role in aerobic energy metabolism. The bioenergetic core functions are executed by 14 central subunits that are conserved ...Mitochondrial complex I is a 1MDa membrane protein complex with a central role in aerobic energy metabolism. The bioenergetic core functions are executed by 14 central subunits that are conserved from bacteria to man. Despite recent progress in structure determination, our understanding of the function of the ~30 accessory subunits associated with the mitochondrial complex is still limited. We have investigated the structure of complex I from the aerobic yeast Yarrowia lipolytica by cryo-electron microscopy. Our density map at 7.9Å resolution closely matches the 3.6-3.9Å X-ray structure of the Yarrowia lipolytica complex. However, the cryo-EM map indicated an additional subunit on the side of the matrix arm above the membrane surface, pointing away from the membrane arm. The density, which is not present in any previously described complex I structure and occurs in about 20 % of the particles, was identified as the accessory sulfur transferase subunit ST1. The Yarrowia lipolytica complex I preparation is active in generating HS from the cysteine derivative 3-mercaptopyruvate, catalyzed by ST1. We thus provide evidence for a link between respiratory complex I and mitochondrial sulfur metabolism.
External linksBiochim Biophys Acta / PubMed:27693469
MethodsEM (single particle)
Resolution7.9 - 9.5 Å
Structure data

EMDB-4110:
Respiratory complex I of Yarrowia lipolytica
Method: EM (single particle) / Resolution: 7.9 Å

EMDB-4111:
Respiratory complex I of Yarrowia lipolytica with sulfur transferase subunit ST1 bound
Method: EM (single particle) / Resolution: 9.5 Å

Source
  • Yarrowia lipolytica (yeast)

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