EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Visualizing the chaperone-mediated folding trajectory of the G protein β5 β-propeller.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 21, Page 3852-33868.e6, Year 2023
掲載日	2023年11月2日
著者	Shuxin Wang / Mikaila I Sass / Yujin Kwon / W Grant Ludlam / Theresa M Smith / Ethan J Carter / Nathan E Gladden / Margot Riggi / Janet H Iwasa / Barry M Willardson / Peter S Shen /
PubMed 要旨	The Chaperonin Containing Tailless polypeptide 1 (CCT) complex is an essential protein folding machine with a diverse clientele of substrates, including many proteins with β-propeller domains. Here, ...The Chaperonin Containing Tailless polypeptide 1 (CCT) complex is an essential protein folding machine with a diverse clientele of substrates, including many proteins with β-propeller domains. Here, we determine the structures of human CCT in complex with its accessory co-chaperone, phosducin-like protein 1 (PhLP1), in the process of folding Gβ, a component of Regulator of G protein Signaling (RGS) complexes. Cryoelectron microscopy (cryo-EM) and image processing reveal an ensemble of distinct snapshots that represent the folding trajectory of Gβ from an unfolded molten globule to a fully folded β-propeller. These structures reveal the mechanism by which CCT directs Gβ folding through initiating specific intermolecular contacts that facilitate the sequential folding of individual β sheets until the propeller closes into its native structure. This work directly visualizes chaperone-mediated protein folding and establishes that CCT orchestrates folding by stabilizing intermediates through interactions with surface residues that permit the hydrophobic core to coalesce into its folded state.
リンク	Mol Cell / PubMed:37852256 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.7 Å
構造データ	40439 8sfe EMDB-40439, PDB-8sfe: Open state CCT-G beta 5 complex 手法: EM (単粒子) / 解像度: 3.36 Å 40440 8sff EMDB-40440, PDB-8sff: CCT G beta 5 complex closed state 0 手法: EM (単粒子) / 解像度: 3.2 Å 40452 8sg8 EMDB-40452, PDB-8sg8: CCT G beta 5 complex closed state 1 手法: EM (単粒子) / 解像度: 3.0 Å 40453 8sg9 EMDB-40453, PDB-8sg9: CCT G beta 5 complex closed state 3 手法: EM (単粒子) / 解像度: 2.9 Å 40454 8sgc EMDB-40454, PDB-8sgc: CCT G beta 5 complex closed state 2 手法: EM (単粒子) / 解像度: 2.9 Å 40461 8sgl EMDB-40461, PDB-8sgl: CCT G beta 5 complex closed state 15 手法: EM (単粒子) / 解像度: 2.9 Å 40464 8sgq EMDB-40464, PDB-8sgq: CCT G beta 5 complex intermediate state 手法: EM (単粒子) / 解像度: 3.7 Å 40481 8sh9 EMDB-40481, PDB-8sh9: CCT G beta 5 complex best PhLP1 class 手法: EM (単粒子) / 解像度: 2.7 Å 40482 8sha EMDB-40482, PDB-8sha: CCT-G beta 5 complex closed state 4 手法: EM (単粒子) / 解像度: 3.0 Å 40484 8shd EMDB-40484, PDB-8shd: CCT G beta 5 complex closed state 10 手法: EM (単粒子) / 解像度: 2.9 Å 40485 8she EMDB-40485, PDB-8she: CCT-G beta 5 complex closed state 8 手法: EM (単粒子) / 解像度: 2.8 Å 40486 8shf EMDB-40486, PDB-8shf: CCT-G beta 5 complex closed state 7 手法: EM (単粒子) / 解像度: 3.0 Å 40487 8shg EMDB-40487, PDB-8shg: CCT G beta 5 complex closed state 9 手法: EM (単粒子) / 解像度: 2.8 Å 40488 8shl EMDB-40488, PDB-8shl: CCT G beta 5 complex closed state 5 手法: EM (単粒子) / 解像度: 3.0 Å 40489 8shn EMDB-40489, PDB-8shn: CCT-G beta 5 complex closed state 6 手法: EM (単粒子) / 解像度: 2.8 Å 40490 8sho EMDB-40490, PDB-8sho: CCT G beta 5 complex close state 11 手法: EM (単粒子) / 解像度: 3.0 Å 40491 8shp EMDB-40491, PDB-8shp: CCT G beta 5 complex closed state 13 手法: EM (単粒子) / 解像度: 3.0 Å 40492 8shq EMDB-40492, PDB-8shq: CCT G beta 5 complex closed state 12 手法: EM (単粒子) / 解像度: 2.9 Å 40494 8sht EMDB-40494, PDB-8sht: CCT G beta 5 complex closed state 14 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-AF3: ALUMINUM FLUORIDE / トリフルオロアルミニウム / フッ化アルミニウム ChemComp-HOH*: WATER / 水
由来	homo sapiens (ヒト)
キーワード	CHAPERONE (シャペロン) / CCT / Gb5 / complex / open