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TitleCorrelative montage parallel array cryo-tomography for in situ structural cell biology.
Journal, issue, pagesNat Methods, Vol. 20, Issue 10, Page 1537-1543, Year 2023
Publish dateSep 18, 2023
AuthorsJie E Yang / Matthew R Larson / Bryan S Sibert / Joseph Y Kim / Daniel Parrell / Juan C Sanchez / Victoria Pappas / Anil Kumar / Kai Cai / Keith Thompson / Elizabeth R Wright /
PubMed AbstractImaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array ...Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substrate micropatterning, and MPACT supports studies that contextually define the three-dimensional architecture of cells. To further extend the flexibility of MPACT, tilt series may be processed in their entirety or as individual tiles suitable for sub-tomogram averaging, enabling efficient data processing and analysis.
External linksNat Methods / PubMed:37723245 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution18.5 - 19.9 Å
Structure data

EMDB-40307: Sub-tomogram average of the RSV F pairs (with over-dosed particles removed) from the surface of native virions released from RSV-infected BEAS-2B cells cultured on EM grids collected via montage parallel array cryo- tomography (MPACT)
Method: EM (subtomogram averaging) / Resolution: 18.5 Å

EMDB-40308: Sub-tomogram average of the RSV F pairs from the surface of native virions released from RSV-infected BEAS-2B cells cultured on EM grids collected via montage parallel array cryo-tomography (MPACT)
Method: EM (subtomogram averaging) / Resolution: 19.9 Å

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