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TitleCryo-EM structure and complementary drug efflux activity of the Acinetobacter baumannii multidrug efflux pump AdeG.
Journal, issue, pagesStructure, Vol. 33, Issue 3, Page 539-551.e4, Year 2025
Publish dateMar 6, 2025
AuthorsZhenlin Ouyang / Wenbo He / Di Wu / Hao An / Lei Duan / Min Jiao / Xiaoyu He / Qinyue Yu / Jiaxin Zhang / Qian Qin / Ruochen Wang / Fang Zheng / Peter M Hwang / Xiaoting Hua / Li Zhu / Yurong Wen /
PubMed AbstractMultidrug-resistant Acinetobacter baumannii has emerged as one of the most antibiotic-resistant bacterial pathogens associated with nosocomial infection, with its resistance highly depending on ...Multidrug-resistant Acinetobacter baumannii has emerged as one of the most antibiotic-resistant bacterial pathogens associated with nosocomial infection, with its resistance highly depending on multiple multidrug efflux pumps. Here, we report the cryoelectron microscopy (cryo-EM) structure of Acinetobacter drug efflux G (AdeG), the inner membrane component of one of three important resistance-nodulation-cell division (RND) pump family members in A. baumannii, which is involved in drug resistance to chloramphenicol, trimethoprim, ciprofloxacin, and clindamycin. We systematically compare the structures and substrate binding specificities of AdeG, AdeB, and AdeJ multidrug efflux pumps via molecular docking, revealing potential determinants for drug binding. Knockout experiments demonstrate a functional complementarity between AdeABC, AdeFGH, and AdeIJK. Our study provides a structural understanding of A. baumannii multidrug efflux pump AdeG and reveals complementary drug efflux activity between AdeG and other RND efflux pumps, which may promote further rational drug discovery efforts targeting multidrug efflux pumps.
External linksStructure / PubMed:39798571
MethodsEM (single particle)
Resolution3.06 Å
Structure data

39532

8yr0

EMDB-39532, PDB-8yr0:
Cryo-EM Structure of AdeG from Acinetobacter baumannii
Method: EM (single particle) / Resolution: 3.06 Å

Chemicals

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

Source
  • acinetobacter baumannii (bacteria)
KeywordsMEMBRANE PROTEIN / Efflux Pump / Acinetobacter baumannii / Multidrug Resistance / AdeG

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