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TitleActivation mechanisms of dimeric mechanosensitive OSCA/TMEM63 channels.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 7504, Year 2024
Publish dateAug 29, 2024
AuthorsYuanyue Shan / Mengmeng Zhang / Meiyu Chen / Xinyi Guo / Ying Li / Mingfeng Zhang / Duanqing Pei /
PubMed AbstractOSCA/TMEM63 channels, which have transporter-like architectures, are bona fide mechanosensitive (MS) ion channels that sense high-threshold mechanical forces in eukaryotic cells. The activation ...OSCA/TMEM63 channels, which have transporter-like architectures, are bona fide mechanosensitive (MS) ion channels that sense high-threshold mechanical forces in eukaryotic cells. The activation mechanism of these transporter-like channels is not fully understood. Here we report cryo-EM structures of a dimeric OSCA/TMEM63 pore mutant OSCA1.1-F516A with a sequentially extracellular dilated pore in a detergent environment. These structures suggest that the extracellular pore sequential dilation resembles a flower blooming and couples to a sequential contraction of each monomer subunit towards the dimer interface and subsequent extrusion of the dimer interface lipids. Interestingly, while OSCA1.1-F516A remains non-conducting in the native lipid environment, it can be directly activated by lyso-phosphatidylcholine (Lyso-PC) with reduced single-channel conductance. Structural analysis of OSCA1.1-F516A in lyso-PC-free and lyso-PC-containing lipid nanodiscs indicates that lyso-PC induces intracellular pore dilation by attracting the M6b to upward movement away from the intracellular side thus extending the intracellular pore. Further functional studies indicate that full activation of MS OSCA/TMEM63 dimeric channels by high-threshold mechanical force also involves the opening of both intercellular and extracellular pores. Our results provide the fundamental activation paradigm of the unique transporter-like MS OSCA/TMEM63 channels, which is likely applicable to functional branches of the TMEM63/TMEM16/TMC superfamilies.
External linksNat Commun / PubMed:39209849 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 2.8 Å
Structure data

39399

8ymm

EMDB-39399, PDB-8ymm:
OSCA1.1-F516A open
Method: EM (single particle) / Resolution: 2.8 Å

39400

8ymn

EMDB-39400, PDB-8ymn:
OSCA1.1-F516A pre-open 2
Method: EM (single particle) / Resolution: 2.5 Å

39401

8ymo

EMDB-39401, PDB-8ymo:
OSCA1.1-F516A pre-open 1
Method: EM (single particle) / Resolution: 2.7 Å

39402

8ymp

EMDB-39402, PDB-8ymp:
OSCA1.1-F516A nanodisc in LPC
Method: EM (single particle) / Resolution: 2.6 Å

39403

8ymq

EMDB-39403, PDB-8ymq:
OSCA1.1-F516A nanodisc
Method: EM (single particle) / Resolution: 2.6 Å

Chemicals

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM

Source
  • arabidopsis thaliana (thale cress)
KeywordsMEMBRANE PROTEIN / OSCA1.1-F516A open / OSCA1.1-F516A pre-open 2 / OSCA1.1-F516A pre-open 1 / OSCA1.1-F516A nanodisc in LPC / OSCA1.1-F516A nanodisc

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