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TitleActivation and inhibition mechanisms of a plant helper NLR.
Journal, issue, pagesNature, Vol. 639, Issue 8054, Page 438-446, Year 2025
Publish dateFeb 12, 2025
AuthorsYinyan Xiao / Xiaoxian Wu / Zaiqing Wang / Kexin Ji / Yang Zhao / Yu Zhang / Li Wan /
PubMed AbstractPlant nucleotide-binding leucine-rich repeat (NLR) receptors sense pathogen effectors and form resistosomes to confer immunity. Some sensor NLR resistosomes produce small molecules to induce ...Plant nucleotide-binding leucine-rich repeat (NLR) receptors sense pathogen effectors and form resistosomes to confer immunity. Some sensor NLR resistosomes produce small molecules to induce formation of a heterotrimer complex with two lipase-like proteins, EDS1 and SAG101, and a helper NLR called NRG1 (refs. ). Activation of sensor NLR resistosomes also triggers NRG1 oligomerization and resistosome formation at the plasma membrane. We demonstrate that the Arabidopsis AtEDS1-AtSAG101-AtNRG1A heterotrimer formation is stabilized by the AtNRG1A loss-of-oligomerization mutant L134E. We report structures of AtEDS1-AtSAG101-AtNRG1A L134E and AtEDS1-AtSAG101-AtNRG1C heterotrimers with similar assembly mechanisms. AtNRG1A signalling is activated by the interaction with the AtEDS1-AtSAG101 heterodimer in complex with their small-molecule ligand. The truncated AtNRG1C maintains core interacting domains of AtNRG1A but develops further interactions with AtEDS1-AtSAG101 to outcompete AtNRG1A. Moreover, AtNRG1C lacks an N-terminal signalling domain and shows nucleocytoplasmic localization, facilitating its sequestration of AtEDS1-AtSAG101, which is also nucleocytoplasmic. Our study shows the activation and inhibition mechanisms of a plant helper NLR.
External linksNature / PubMed:39939758
MethodsEM (single particle)
Resolution3.1 Å
Structure data

39383

8yl6

EMDB-39383, PDB-8yl6:
EDS1-SAG101-NRG1A L134E heterotrimer
Method: EM (single particle) / Resolution: 3.1 Å

39384

8yl7

EMDB-39384, PDB-8yl7:
EDS1-SAG101-NRG1C heterotrimer
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

PDB-1l15:
CONTRIBUTIONS OF HYDROGEN BONDS OF THR 157 TO THE THERMODYNAMIC STABILITY OF PHAGE T4 LYSOZYME

Source
  • arabidopsis thaliana (thale cress)
KeywordsIMMUNE SYSTEM/HYDROLASE / Effector-triggered immunity (ETI) / Enhanced Disease Susceptibility1 / Senescence-Associated Gene101 / N Required Gene 1.1 / IMMUNE SYSTEM / IMMUNE SYSTEM-HYDROLASE complex / N Required Gene 1.3

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