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TitleCombined H-Detected Solid-State NMR Spectroscopy and Electron Cryotomography to Study Membrane Proteins across Resolutions in Native Environments.
Journal, issue, pagesStructure, Vol. 26, Issue 1, Page 161-170.e3, Year 2018
Publish dateJan 2, 2018
AuthorsLindsay A Baker / Tessa Sinnige / Pascale Schellenberger / Jeanine de Keyzer / C Alistair Siebert / Arnold J M Driessen / Marc Baldus / Kay Grünewald /
PubMed AbstractMembrane proteins remain challenging targets for structural biology, despite much effort, as their native environment is heterogeneous and complex. Most methods rely on detergents to extract membrane ...Membrane proteins remain challenging targets for structural biology, despite much effort, as their native environment is heterogeneous and complex. Most methods rely on detergents to extract membrane proteins from their native environment, but this removal can significantly alter the structure and function of these proteins. Here, we overcome these challenges with a hybrid method to study membrane proteins in their native membranes, combining high-resolution solid-state nuclear magnetic resonance spectroscopy and electron cryotomography using the same sample. Our method allows the structure and function of membrane proteins to be studied in their native environments, across different spatial and temporal resolutions, and the combination is more powerful than each technique individually. We use the method to demonstrate that the bacterial membrane protein YidC adopts a different conformation in native membranes and that substrate binding to YidC in these native membranes differs from purified and reconstituted systems.
External linksStructure / PubMed:29249608 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution50.0 - 100.0 Å
Structure data

EMDB-3909:
Stalled E. coli ribosomes (Fo-c SecM nascent chains) and native E. coli membranes containing recombinant YidC
Method: EM (subtomogram averaging) / Resolution: 50.0 Å

EMDB-3919:
Stalled E. coli ribosomes (Fo-c SecM nascent chains) and native E. coli membranes containing recombinant YidC
Method: EM (subtomogram averaging) / Resolution: 100.0 Å

EMDB-3920:
Stalled E. coli ribosomes (Fo-c SecM nascent chains) and native E. coli membranes containing recombinant YidC
Method: EM (subtomogram averaging) / Resolution: 100.0 Å

EMDB-3921:
Stalled E. coli ribosomes (Fo-c SecM nascent chains) and native E. coli membranes containing recombinant YidC
Method: EM (subtomogram averaging) / Resolution: 100.0 Å

EMDB-3922:
Stalled E. coli ribosomes (Fo-c SecM nascent chains) and native E. coli membranes containing recombinant YidC
Method: EM (subtomogram averaging) / Resolution: 100.0 Å

EMDB-3923:
Stalled E. coli ribosomes (Fo-c SecM nascent chains) and native E. coli membranes containing recombinant YidC
Method: EM (subtomogram averaging) / Resolution: 100.0 Å

EMDB-3924:
Stalled E. coli ribosomes (Fo-c SecM nascent chains) and native E. coli membranes containing recombinant YidC
Method: EM (subtomogram averaging) / Resolution: 100.0 Å

Source
  • Escherichia coli (E. coli)

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